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DNA lesions in hyperthermic cell killing: effects of thermotolerance, procaine, and erythritol

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576564· OSTI ID:5615538
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When HeLa S3 cells were subjected to 45 degrees C hyperthermia, DNA lesions were detected by the use of the alkaline unwinding/hydroxylapatite method. The number of lesions formed was not affected when the cells were made thermotolerant by either an acute (15 min 44 degrees C + 5 h 37 degrees C) or a chronic (5 h 42 degrees C) pretreatment before 45 degrees C hyperthermia. The presence of 10 mM procaine (heat sensitizer) or 0.5 M erythritol (heat protector) during hyperthermia also had no effect on the rate of formation of heat-induced alkali labile DNA lesions. These observations do not support a concept where DNA lesions are considered to be the ultimate cause of hyperthermic cell killing. Both drugs, however, influenced the rate of repair of radiation-induced strand breaks when present during preirradiation heat treatment. We conclude that the initial number of heat-induced alkali labile DNA lesions is not directly related to cell survival. It cannot be excluded, however, that differences in posthyperthermic repair of these lesions may lead to a positive correlation between residual DNA damage and survival after the different experimental conditions.
Research Organization:
State Univ. of Groningen, The Netherlands
OSTI ID:
5615538
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 1; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560201* -- Thermal Effects-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANESTHETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY TEMPERATURE
CARBOHYDRATES
CELL KILLING
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DNA
DNA REPAIR
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ENERGY
ERYTHRITOL
HEAT
HELA CELLS
HYDROXY COMPOUNDS
HYPERTHERMIA
MONOSACCHARIDES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROCAINE
RECOVERY
REPAIR
SACCHARIDES
STRAND BREAKS
SURVIVAL TIME
TEMPERATURE EFFECTS