DNA polymerase activity in heat killing and hyperthermic radiosensitization of mammalian cells as observed after fractionated heat treatments

Journal Article · Sat Mar 01 04:00:00 EST 1986 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576687· OSTI ID:5737133

Jorritsma, J B; Burgman, P; Kampinga, H H; Konings, A W

Possible relations between hyperthermic inactivation of alpha and beta DNA polymerase activity and hyperthermic cell killing or hyperthermic radiosensitization were investigated. Ehrlich Ascites Tumor (EAT) cells and HeLa S3 cells were treated with fractionated doses of hyperthermia. The heating schedules were chosen such that the initial heat treatment resulted in either thermotolerance or thermosensitization (step-down heating) for the second heat treatment. The results show that for DNA polymerase activity and heat radiosensitization (cell survival) no thermotolerance or thermosensitization is observed. Thus hyperthermic cell killing and DNA polymerase activity are not correlated. The correlation of hyperthermic radiosensitization and DNA polymerase activity was substantially less than observed in previous experiments with normotolerant and thermotolerant HeLa S3 cells. We conclude that alpha and beta DNA polymerase inactivation is not always the critical cellular process responsible for hyperthermic cell killing or hyperthermic radiosensitization. Other possible cellular systems that might determine these processes are discussed.

• Research Organization: State Univ. of Groningen, The Netherlands
• OSTI ID: 5737133
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560201* -- Thermal Effects-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ASCITES TUMOR CELLS
BODY TEMPERATURE
CELL KILLING
DNA POLYMERASES
EHRLICH ASCITES TUMOR
ENERGY
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL NEOPLASMS
HEAT
HELA CELLS
HYPERTHERMIA
NUCLEOTIDYLTRANSFERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
SENSITIVITY
SURVIVAL TIME
TEMPERATURE EFFECTS
TRANSFERASES
TUMOR CELLS