Effects of hyperthermia on DNA polymerases alpha and beta: relationship to thermal cell killing and radiosensitization

Journal Article · Tue Jun 01 04:00:00 EDT 1982 · NIDA Res. Monogr.; (United States)

OSTI ID:5993331

Denman, D L; Spiro, I J; Dewey, W C

The relationship between heat-induced reduction in the activity of the alpha and beta DNA polymerases and both hyperthermic cell killing and radiosensitization was investigated with cultured Chinese hamster ovary cells. Positive correlations were observed between the reduction in beta-polymerase activity and the fraction of cells surviving a given heat exposure. Also, thermal tolerance was observed both for reduction in beta-polymerase and for cell killing. In addition, as the time between heating and a subsequent exposure to X-irradiation increased, both beta-polymerase activity and cell survival increased.

• Research Organization: Department of Radiology and Radiation Biology, Colorado State University, Ft. Collins
• OSTI ID: 5993331
• Journal Information: NIDA Res. Monogr.; (United States), Journal Name: NIDA Res. Monogr.; (United States) Vol. 61; ISSN MIDAD
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560201 -- Thermal Effects-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BODY TEMPERATURE
CELL CULTURES
CHEMICAL ANALYSIS
DNA
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
HAMSTERS
HYPERTHERMIA
IONIZING RADIATIONS
MAMMALS
NORTH AMERICA
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
QUANTITATIVE CHEMICAL ANALYSIS
RADIATIONS
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RODENTS
SURVIVAL CURVES
TEMPERATURE EFFECTS
TOLERANCE
TRANSFERASES
USA
VERTEBRATES
X RADIATION