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Development of cytochemical markers for cell transformation and carcinogenesis

Book ·
OSTI ID:5606107
; ; ;
The objective of this program is to develop rapid, sensitive, and economical systems for the in vitro and cytological assay for carcinogenic effects of substances involved in the extraction, conversion and utilization of nonnuclear energy sources, with particular consideration of in situ coal gasification, shale-oil utilization, coal-burning power plants, and geothermal power plants. The approach is based on the development of cytochemical markers for cell transformation, and on our ability to quantitate such markers by microfluorometry ad by flow systems analysis and sorting. The intracellular levels of several hydrolytic enzymes have been chosen as potential early indicators of cell transformation. Using synthetic fluorogenic enzyyme subtrates, the levels of four proteases and five esterases have been measured on monolayer cultures of Chinese Hamster Ovary cells, Syrian hamster embryo cells and human lung fibroblasts (both normal and viral transformed). Hydrolase profiles generated from these measurements indicate significant differences between cell types. Flow cytometry analysis on WI38 cells (normal versus SV-40 transformed) indicates that intracellular Cathepsin B1 and alkaline phosphatase levels can be used to distinguish individual normal cells from transformed cells. These same enzyme assays will be used to determine an optimum in-vitro system for subsequent carcinogen treatment and flow analysis.
OSTI ID:
5606107
Country of Publication:
United States
Language:
English

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Related Subjects

01 COAL, LIGNITE, AND PEAT
016000 -- Coal
Lignite
& Peat-- Health & Safety
04 OIL SHALES AND TAR SANDS
040700 -- Oil Shales & Tar Sands-- Health & Safety
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE PHOSPHATASE
ANIMAL CELLS
ANIMALS
BIOLOGICAL INDICATORS
CARCINOGEN SCREENING
CARCINOGENESIS
CELL FLOW SYSTEMS
COAL GASIFICATION
CONNECTIVE TISSUE CELLS
EMISSION SPECTROSCOPY
ENERGY SOURCES
ENZYME ACTIVITY
ENZYMES
ESTERASES
FIBROBLASTS
FLUORESCENCE SPECTROSCOPY
FOSSIL FUELS
FOSSIL-FUEL POWER PLANTS
FUELS
GASIFICATION
GEOTHERMAL POWER PLANTS
HAMSTERS
HYDROLASES
IN-SITU GASIFICATION
IN-SITU PROCESSING
MAMMALS
MAN
MEASURING METHODS
MINERAL OILS
OILS
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
PATHOGENESIS
PHOSPHATASES
POWER PLANTS
PRIMATES
PROCESSING
RODENTS
SCREENING
SHALE OIL
SOMATIC CELLS
SORTING
SPECTROSCOPY
THERMAL POWER PLANTS
THERMOCHEMICAL PROCESSES
VERTEBRATES