Flow cytoenzymology: a tool in the search for neoplastic markers
Conference
·
OSTI ID:7027233
The rapid determination of enzyme activity in single cells by flow microfluorometry (flow cytoenzymology) is described. Cells are routinely analyzed for 10 to 12 enzymes. When two populations differ in profile, e.g., normal and neoplastic cells, then a suitable fluorogenic substrate permits flow cytometric assay of the activity. Cells that differ in an enzyme or isozyme may be discriminated and sorted electronically for further analysis. The discrimination and sorting of transformed human lung fibroblast from nontransformed fibroblasts by an arylamidase activity demonstrates this technology. The sorted cell types were identified further by a second marker, the Regan isozyme of alkaline phosphatase. The application of flow cytoenzymology to the assay of neoplastic markers is discussed.
- Research Organization:
- California Univ., Livermore (USA). Lawrence Livermore Lab.
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 7027233
- Report Number(s):
- UCID-81202; CONF-780576-1
- Country of Publication:
- United States
- Language:
- English
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