Early polymorphonuclear leukocyte accumulation correlates with the development of posttraumatic cerebral edema in rats

Journal Article · · Journal of Neurotrauma; (USA)

DOI:https://doi.org/10.1089/neu.1990.7.207· OSTI ID:5602886

Schoettle, R J; Kochanek, P M; Magargee, M J; Uhl, M W; Nemoto, E M ^[1]

---

1. Univ. of Pittsburgh, PA (USA)

To evaluate the role of polymorphonuclear leukocytes (PMNs) in the development of posttraumatic cerebral edema, we quantitatively assessed the time course and magnitude of PMN accumulation and its relationship to cerebral edema formation after cerebral trauma in 78 rats. {sup 111}In-labeled PMN accumulation was measured in 26 rats in the first 8 h after right hemispheric percussive cerebral trauma or a sham control condition. {sup 51}Cr-labeled erythrocyte accumulation was measured simultaneously in 22 rats to assess the contribution of expansion of blood volume to early posttraumatic PMN accumulation. Edema formation (right-left (R-L) hemispheric difference in percent brain water), R-L hemispheric labeled-PMN accumulation, and blood volume index-adjusted PMN accumulation were measured between 0-2 h and 4-8 h posttrauma. PMN accumulation was elevated markedly in the first 2 h posttrauma compared with values in sham controls (13.45 +/- 2.53 vs -0.03 +/- 0.31, p less than 0.01) but not when adjusted for blood volume index (BVI), suggesting that PMN accumulation in the first 2 h posttrauma was due to expansion of blood volume. Between 4 and 8 h posttrauma, however, both total (2.56 +/- 0.82 vs -0.29 +/- 0.52) and BVI-adjusted (8.78 +/- 3.97 vs -0.48 +/- 0.79) PMN accumulation were elevated (p less than 0.05) compared with sham. Brain edema and total PMN accumulation were significantly correlated at both 2 h and 8 h posttrauma (r2 = 0.77, p less than 0.001, and r2 = 0.69, p less than 0.002, respectively), but a significant correlation between edema and BVI-adjusted PMN accumulation was observed only at 8 h posttrauma (r2 = 0.96, p less than 0.001). These data show that PMN accumulation after traumatic brain injury occurs with an initial phase explained by an increase in blood volume in the first 2 h posttrauma followed by a subsequent acute inflammatory phase.

• OSTI ID: 5602886
• Journal Information: Journal of Neurotrauma; (USA), Vol. 7:4; ISSN 0897-7151
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CEREBRAL CORTEX
EDEMA
NERVOUS SYSTEM DISEASES
PATHOGENESIS
NEUTROPHILS
BIOLOGICAL FUNCTIONS
CHROMIUM 51
ERYTHROCYTES
INDIUM 111
OXYGEN
RATS
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BRAIN
CENTRAL NERVOUS SYSTEM
CEREBRUM
CHROMIUM ISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NERVOUS SYSTEM
NONMETALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PATHOLOGICAL CHANGES
RADIOISOTOPES
RODENTS
SYMPTOMS
VERTEBRATES
550901* - Pathology- Tracer Techniques