Early polymorphonuclear leukocyte accumulation correlates with the development of posttraumatic cerebral edema in rats

Journal Article · · Journal of Neurotrauma; (USA)

DOI:https://doi.org/10.1089/neu.1990.7.207· OSTI ID:5602886

Schoettle, R J; Kochanek, P M; Magargee, M J; Uhl, M W; Nemoto, E M ^[1]

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1. Univ. of Pittsburgh, PA (USA)

To evaluate the role of polymorphonuclear leukocytes (PMNs) in the development of posttraumatic cerebral edema, we quantitatively assessed the time course and magnitude of PMN accumulation and its relationship to cerebral edema formation after cerebral trauma in 78 rats. {sup 111}In-labeled PMN accumulation was measured in 26 rats in the first 8 h after right hemispheric percussive cerebral trauma or a sham control condition. {sup 51}Cr-labeled erythrocyte accumulation was measured simultaneously in 22 rats to assess the contribution of expansion of blood volume to early posttraumatic PMN accumulation. Edema formation (right-left (R-L) hemispheric difference in percent brain water), R-L hemispheric labeled-PMN accumulation, and blood volume index-adjusted PMN accumulation were measured between 0-2 h and 4-8 h posttrauma. PMN accumulation was elevated markedly in the first 2 h posttrauma compared with values in sham controls (13.45 +/- 2.53 vs -0.03 +/- 0.31, p less than 0.01) but not when adjusted for blood volume index (BVI), suggesting that PMN accumulation in the first 2 h posttrauma was due to expansion of blood volume. Between 4 and 8 h posttrauma, however, both total (2.56 +/- 0.82 vs -0.29 +/- 0.52) and BVI-adjusted (8.78 +/- 3.97 vs -0.48 +/- 0.79) PMN accumulation were elevated (p less than 0.05) compared with sham. Brain edema and total PMN accumulation were significantly correlated at both 2 h and 8 h posttrauma (r2 = 0.77, p less than 0.001, and r2 = 0.69, p less than 0.002, respectively), but a significant correlation between edema and BVI-adjusted PMN accumulation was observed only at 8 h posttrauma (r2 = 0.96, p less than 0.001). These data show that PMN accumulation after traumatic brain injury occurs with an initial phase explained by an increase in blood volume in the first 2 h posttrauma followed by a subsequent acute inflammatory phase.

• OSTI ID: 5602886
• Journal Information: Journal of Neurotrauma; (USA), Journal Name: Journal of Neurotrauma; (USA) Vol. 7:4; ISSN 0897-7151; ISSN JNEUE
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BRAIN
CENTRAL NERVOUS SYSTEM
CEREBRAL CORTEX
CEREBRUM
CHROMIUM 51
CHROMIUM ISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
EDEMA
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ERYTHROCYTES
EVEN-ODD NUCLEI
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NERVOUS SYSTEM
NERVOUS SYSTEM DISEASES
NEUTROPHILS
NONMETALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
OXYGEN
PATHOGENESIS
PATHOLOGICAL CHANGES
RADIOISOTOPES
RATS
RODENTS
SYMPTOMS
TRACER TECHNIQUES
VERTEBRATES