Cytophotometric studies of DNA in circulating erythrocytes of Brook trout exposed to acid pH

Journal Article · Tue Dec 31 23:00:00 EST 1968 · Proc. Pa. Acad. Sci.; (United States)

OSTI ID:5602342

Vaala, S S; Mitchell, R B; Anthony, A

Trout exposed to low pH (3.5) for four days accumulate excessive p.a.s.-positive mucin notably in the gill region. This is known to interfere with oxygen diffusion across gill filaments leading to hypoxemia and death. To determine whether any augmentation of hemopoiesis occurs in such trout, quantitative analyses were made of nuclear fuelgen-DNA profiles of circulating populations of erythrocytes using the two-wavelength method of microspectrophotometry. No evidence was found of either enhanced hemopoiesis or increased rate of red cell destruction in acid-water exposed fish. These data suggest that one of the reasons why trout die in acid water is that compensatory erythropoiesis does not occur or is not as rapidly initiated in fish as in homeotherms in response to oxygen lack.

• OSTI ID: 5602342
• Journal Information: Proc. Pa. Acad. Sci.; (United States), Journal Name: Proc. Pa. Acad. Sci.; (United States) Vol. 43; ISSN PPASA
• Country of Publication: United States
• Language: English

Related Subjects

520200 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID RAIN
ACIDIFICATION
ANIMALS
AQUATIC ORGANISMS
ATMOSPHERIC PRECIPITATIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DEATH
DNA
ERYTHROCYTES
FISHES
GILLS
LAKES
MATERIALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PH VALUE
POLLUTION
RAIN
RESPIRATORY SYSTEM
SPECTROPHOTOMETRY
SURFACE WATERS
TOXICITY
TROUT
VERTEBRATES
WATER POLLUTION