Victorin-induced callose in mesophyll protoplasts of Avena sativa
Conference
·
· Plant Physiology, Supplement; (United States)
OSTI ID:5601094
- Michigan State Univ. Plant Research Lab., East Lansing (United States)
Callose ((1-3){beta}-D-glucan), measured as incorporation of {sup 14}C-glucose into ethanol-insoluble product, is produced within 2h in victorin treated mesophyll protoplasts of victorin-sensitive cultivars of oat (Avena sativa). This production is ten-fold higher in the presence of 6 ng victorin/ml than untreated protoplasts after 2h. Microsomes of victorin-treated and untreated protoplasts are assayed for callose synthase activity using radiolabeled substrate, UDP-glucose. Preliminary studies indicate that microsomes of victorin-treated protoplasts have up to four times more callose synthase activity than microsomes of untreated protoplasts. Therefore, stimulation of callose synthase by some means that survives microsome isolation, at least in part, may account for the effect of victorin.
- DOE Contract Number:
- AC02-76ER01338
- OSTI ID:
- 5601094
- Report Number(s):
- CONF-9107184--
- Conference Information:
- Journal Name: Plant Physiology, Supplement; (United States) Journal Volume: 96:1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Victorin-induced extracellular. beta. -glucan in oat protoplasts
Aluminum ions induce oat protoplasts to produce an extracellular (1 yields 3). beta. -D-glucan
Direct photolabeling with ( sup 32 P)UDP-glucose for identification of a subunit of cotton fiber callose synthase. [Gossypium hirsutum]
Conference
·
Tue May 01 00:00:00 EDT 1990
· Plant Physiology, Supplement; (USA)
·
OSTI ID:5839578
Aluminum ions induce oat protoplasts to produce an extracellular (1 yields 3). beta. -D-glucan
Journal Article
·
Sat Sep 01 00:00:00 EDT 1990
· Plant Physiology; (USA)
·
OSTI ID:5922099
Direct photolabeling with ( sup 32 P)UDP-glucose for identification of a subunit of cotton fiber callose synthase. [Gossypium hirsutum]
Journal Article
·
Thu Jan 31 23:00:00 EST 1991
· Plant Physiology; (United States)
·
OSTI ID:6056828
Related Subjects
551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANTIGENS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CELL CONSTITUENTS
CEREALS
ENZYMES
GLUCOSE
GLYCOSYL TRANSFERASES
GRAMINEAE
HAZARDOUS MATERIALS
HEXOSES
KINETICS
LABELLED COMPOUNDS
LILIOPSIDA
MAGNOLIOPHYTA
MATERIALS
MICROSOMES
MONOSACCHARIDES
OATS
ORGANIC COMPOUNDS
PHYSIOLOGY
PLANT CELLS
PLANTS
POLYSACCHARIDES
PROTEINS
REACTION KINETICS
RIBOSOMES
SACCHARIDES
TOXIC MATERIALS
TOXINS
TRANSFERASES
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANTIGENS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CELL CONSTITUENTS
CEREALS
ENZYMES
GLUCOSE
GLYCOSYL TRANSFERASES
GRAMINEAE
HAZARDOUS MATERIALS
HEXOSES
KINETICS
LABELLED COMPOUNDS
LILIOPSIDA
MAGNOLIOPHYTA
MATERIALS
MICROSOMES
MONOSACCHARIDES
OATS
ORGANIC COMPOUNDS
PHYSIOLOGY
PLANT CELLS
PLANTS
POLYSACCHARIDES
PROTEINS
REACTION KINETICS
RIBOSOMES
SACCHARIDES
TOXIC MATERIALS
TOXINS
TRANSFERASES