Direct photolabeling with ( sup 32 P)UDP-glucose for identification of a subunit of cotton fiber callose synthase. [Gossypium hirsutum]
Journal Article
·
· Plant Physiology; (United States)
- Hebrew Univ., Jerusalem (Israel)
The authors have identified a 52 kilodalton polypeptide as being a likely candidate for the catalytic subunit of the UDP-glucose: (1{r arrow}3)-{beta}-glucan (callose) synthase of developing fibers of Gossypium hirsutum (cotton). Such a polypeptide migrates coincident with callose synthase during glycerol gradient centrifugation in the presence of EDTA, and can be directly photolabeled with the radioactive substrate, {alpha}-({sup 32}P)UDP-glucose. Interaction with the labeled probe requires Ca{sup 2+}, a specific activator of callose synthase which is known to lower the K{sub m} of higher plant callose synthases for the substrate UDP-glucose. Using this probe and several other related ones, several other proteins which interact with UDP-glucose were also identified, but none satisfied all of the above criteria for being components of the callose synthase.
- DOE Contract Number:
- AC02-76ER01338
- OSTI ID:
- 6056828
- Journal Information:
- Plant Physiology; (United States), Journal Name: Plant Physiology; (United States) Vol. 95:2; ISSN 0032-0889; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM COMPOUNDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CATIONS
CHARGED PARTICLES
CHELATING AGENTS
COTTON PLANTS
DAYS LIVING RADIOISOTOPES
EDTA
ENZYME ACTIVITY
ENZYMES
GLUCOSE
GLYCOSYL TRANSFERASES
HEXOSES
IONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLANTS
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
SACCHARIDES
TRACER TECHNIQUES
TRANSFERASES
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM COMPOUNDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CATIONS
CHARGED PARTICLES
CHELATING AGENTS
COTTON PLANTS
DAYS LIVING RADIOISOTOPES
EDTA
ENZYME ACTIVITY
ENZYMES
GLUCOSE
GLYCOSYL TRANSFERASES
HEXOSES
IONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLANTS
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
SACCHARIDES
TRACER TECHNIQUES
TRANSFERASES