Stretches of oligogalacturonan are present in the backbone of rhamnogalacturonan I from cotton suspension culture cell walls
- Oklahoma State Univ., Stillwater (United States)
A polysaccharide fraction, solubilized from cotton suspension culture cell walls by sequential treatments by endopolygalacturonase and cellulase and purified by gel filtration chromatography, contained the sugars characteristic of rhamnogalacturonan I (RGI). A known structural feature of the backbone of RGI is the repeating disaccharide galA-rha; however, the ratio of galA to rha residues was found to be 1 {center dot} 9:1. Treatment of the RGI preparation with liquid HF (containing 1% water) at {minus}23{degree} caused cleavage of most of the rhamnosyl linkages without cleavage of galacturonosyl linkages. Neutral sugar linkages of the sidechains were also mostly cleaved. Characterization of the products from the backbone by nmr spectroscopy, compositional analysis, and liquid secondary ion mass spectrometry suggested that the backbone of the RGI is made up of short segments ({approximately} 10 units) of the galA-rha repeat interspersed with short homogalacturonan segments of {approximately} 10 galA residues of which about 40% are methyl esterified. The sequential enzyme treatments only solubilize {approximately} 40% of the RGI. The residual RGI contains a higher proportion of falA-rha repeats in its backbone.
- DOE Contract Number:
- FG05-86ER13496
- OSTI ID:
- 5601021
- Report Number(s):
- CONF-9107184--
- Journal Information:
- Plant Physiology, Supplement; (United States), Journal Name: Plant Physiology, Supplement; (United States) Vol. 96:1; ISSN PPYSA; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CARBOHYDRATES
CELL CONSTITUENTS
CELL CULTURES
CELL WALL
CELLULASE
CHEMICAL COMPOSITION
COTTON PLANTS
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PHYSIOLOGY
PLANTS
POLYSACCHARIDES
PROTEINS
SACCHARIDES