Structure of plant cell walls. X. Rhamnogalacturonan I, a structurally complex pectic polysaccharide in the walls of suspension-cultured sycamore cells
The purification and characterization of a pectic polymer, rhamnogalacturonan I, present in the primary cell walls of dicots is described. Rhamnogalacturonan I accounts for approximately 7% of the mass of the walls isolated from suspension-cultured sycamore cells. As purified, rhamnogalacturonan I has a molecular weight of approximately 200,000 and is composed primarily of L-rhamnosyl, D-galacturonosyl, L-arabinosyl, and D-galactosyl residues. The backbone of rhamnogalacturonan I is thought to be composed predominantly of D-galacturonosyl and L-rhamnosyl residues in a ratio of approximately 2:1. About half of the L-rhamnosyl residues are 2-linked and are glycosidically attached to C/sub 4/ of a D-galacturonosyl residue. The other half of the L-rhamnosyl residues are 2,4-linked and have a D-galacturonosyl residue glycosidically attached at C/sub 2/. Sidechains averaging 6 residues in length are attached to C/sub 4/ of the L-rhamnosyl residues. There are many different sidechains, containing variously linked L-arabinosyl, and/or D-galactosyl residues.
- Research Organization:
- Univ. of Colorado, Boulder
- OSTI ID:
- 6603057
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 66:6; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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550800* -- Morphology
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
BIOASSAY
BIOCHEMISTRY
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL WALL
CHEMICAL ANALYSIS
CHEMISTRY
CHROMATOGRAPHY
CONFIGURATION
DATA
ENZYMES
GALACTURONIC ACID
HYDROXY ACIDS
INFORMATION
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
MORPHOLOGY
ORGANIC ACIDS
ORGANIC COMPOUNDS
PECTINS
PLANT CELLS
PLANTS
POLYSACCHARIDES
QUANTITATIVE CHEMICAL ANALYSIS
SACCHARIDES
SEPARATION PROCESSES
STRUCTURAL CHEMICAL ANALYSIS
SYCAMORES
TREES