Use of oxygen-18 isotopic labeling to assay photorespiration in terrestrial plants and algae

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:5600343

de Veau, E J

A new method was devised to quantify photorespiration. The assay utilized {sup 18}O{sub 2} to isotopically label intermediates of the glycolate pathway, specifically glycolate, glycine, and serine, for various time periods. The pathway intermediates were isolated and analyzed on a mass spectrometer to determine molecular percent {sup 18}O-enrichment. Rates of glycolate synthesis were determined from: {sup 18}O-labeling kinetics of the intermediates, derived rate equations, and non-linear regression techniques. The method was adapted to measure photorespiratory rates in both terrestrial plants and algae. Test plants are Triticum aestivum, Zea mays L., Pavlova lutheri and Chlorella pyrenoidosa.

• Research Organization: Pennsylvania State Univ., University Park, PA (USA)
• OSTI ID: 5600343
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALGAE
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CEREALS
CHEMICAL REACTIONS
CHLORELLA
CHLOROPHYCOTA
EVEN-EVEN NUCLEI
FUNCTIONS
GLYCOLIC ACID
GRAMINEAE
HYDROXY ACIDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LILIOPSIDA
MAGNOLIOPHYTA
MAIZE
MASS SPECTROSCOPY
MATHEMATICS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN 18
OXYGEN ISOTOPES
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PLANTS
RADIOASSAY
REGRESSION ANALYSIS
SPECTROSCOPY
STABLE ISOTOPES
STATISTICS
SYNTHESIS
TRACER TECHNIQUES
UNICELLULAR ALGAE
WHEAT