Integrated flux and pool size analysis in plant central metabolism reveals unique roles of glycine and serine during photorespiration

Fu, Xinyu; Gregory, Luke M.; Weise, Sean E.; Walker, Berkley J.

doi:10.1038/s41477-022-01294-9

Integrated flux and pool size analysis in plant central metabolism reveals unique roles of glycine and serine during photorespiration

Journal Article · Mon Dec 19 00:00:00 EST 2022 · Nature Plants (Online)

DOI:https://doi.org/10.1038/s41477-022-01294-9· OSTI ID:1970319

Fu, Xinyu ^[1]; ^[2]; Weise, Sean E. ^[2]; ^[2]

Michigan State Univ., East Lansing, MI (United States); Michigan State University Department of Plant Biology
Michigan State Univ., East Lansing, MI (United States)

Photorespiration is an essential process juxtaposed between plant carbon and nitrogen metabolism that responds to dynamic environments. Photorespiration recycles inhibitory intermediates arising from oxygenation reactions catalysed by Rubisco back into the C₃ cycle, but it is unclear what proportions of its nitrogen-containing intermediates (glycine and serine) are exported into other metabolisms in vivo and how these pool sizes affect net CO₂ gas exchange during photorespiratory transients. Here, to address this uncertainty, we measured rates of amino acid export from photorespiration using isotopically non-stationary metabolic flux analysis. This analysis revealed that ~23–41% of the photorespiratory carbon was exported from the pathway as serine under various photorespiratory conditions. Furthermore, we determined that the build-up and relaxation of glycine pools constrained a large portion of photosynthetic acclimation during photorespiratory transients. Furthermore, these results reveal the unique and important roles of glycine and serine in successfully maintaining various photorespiratory fluxes that occur under environmental fluctuations in nature and providing carbon and nitrogen for metabolism.

View Accepted Manuscript (DOE)

Research Organization:: Great Lakes Bioenergy Research Center, Madison, WI (United States); Michigan State Univ., East Lansing, MI (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Contributing Organization:: MSU Institute for Cyber-Enabled Research Cloud Computing Fellowship

Grant/Contract Number:: FG02-91ER20021; SC0018409

OSTI ID:: 1970319

Journal Information:: Nature Plants (Online), Journal Name: Nature Plants (Online) Journal Issue: 1 Vol. 9; ISSN 2055-0278

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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