Metabolic flux analysis in leaf metabolism quantifies the link between photorespiration and one carbon metabolism

Gashu, Kelem; Kaste, Joshua A. M.; Roje, Sanja; Walker, Berkley J.

doi:10.1038/s41477-025-02091-w

Metabolic flux analysis in leaf metabolism quantifies the link between photorespiration and one carbon metabolism

Journal Article · Wed Sep 03 00:00:00 EDT 2025 · Nature Plants

DOI:https://doi.org/10.1038/s41477-025-02091-w· OSTI ID:2998731

Gashu, Kelem ^[1]; ^[2]; Roje, Sanja ^[3]; ^[1]

Michigan State University, East Lansing, MI (United States)
University of Illinois Urbana-Champaign, IL (United States)
Washington State University, Pullman, WA (United States)

Photorespiration is the second largest carbon flux in most leaves and is integrated into metabolism broadly including one-carbon (C₁) metabolism. Photorespiratory intermediates such as serine and others may serve as sources of C₁ units, but it is unclear to what degree this happens in vivo, whether altered photorespiration changes flux to C₁ metabolism, and if so through which intermediates. To clarify these questions, we quantified carbon flux from photorespiration to C₁ metabolism using ¹³CO₂ labelling and isotopically non-stationary metabolic flux analysis in Arabidopsis thaliana under different O₂ concentrations which modulate photorespiration. The results revealed that ~5.8% of assimilated carbon passes to C₁ metabolism under ambient photorespiratory conditions, but this flux greatly decreases under limited photorespiration. Furthermore, the primary carbon flux from photorespiration to C₁ metabolism is through serine. Our results provide fundamental insight into how photorespiration is integrated into C₁ metabolism, with possible implications for C₁ metabolic response to climate change.

View Accepted Manuscript (DOE)

Research Organization:: Michigan State University, East Lansing, MI (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: FG02-91ER20021

OSTI ID:: 2998731

Alternate ID(s):: OSTI ID: 2998777

Journal Information:: Nature Plants, Journal Name: Nature Plants Journal Issue: 9 Vol. 11; ISSN 2055-0278

Publisher:: Springer Science and Business Media LLCCopyright Statement

Country of Publication:: United States

Language:: English

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