Comparison of two isotope dilution/mass spectrometric methods for determination of total serum cholesterol

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5594116

Schaffer, R; Sniegoski, L T; Welch, M J; White, V E; Cohen, A; Hertz, H S; Mandel, J; Paule, R C; Svensson, L; Bjoerkhem, I; Blomstrand, R

Isotope dilution/mass spectrometric methods for total serum cholesterol, developed separately at the Karolinska Institutet (KI) and the National Bureau of Standards (NBS), were compared by applying them to a common set of serum pools. A search for the cause of a systematic difference of a few percent in results from the two methods revealed that the KI cholesterol standard contained lathosterol, which interfered with the calibration of the method. With NBS Standard Reference Material cholesterol used for new analyses at the KI, the average difference in mean values dropped to 0.2%. The NBS results are more precise. This is attributed to the protocols NBS used for sample preparation and mass spectrometry. However, these protocols make the NBS method more complex and time-consuming. A recent critical article on the use of this technique for total cholesterol is also examined.

• Research Organization: Center for Analytical Chemistry, National Bureau of Standards, Washington, DC
• OSTI ID: 5594116
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 28:1; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
550201 -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
CALIBRATION STANDARDS
CHOLESTEROL
COMPARATIVE EVALUATIONS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
MASS SPECTROSCOPY
NATIONAL ORGANIZATIONS
ORGANIC COMPOUNDS
SAMPLE PREPARATION
SPECTROSCOPY
STANDARDS
STEROIDS
STEROLS
TRACER TECHNIQUES
US DOC
US NBS
US ORGANIZATIONS