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Induction of mutations in extracellular lambda phage by gamma radiation: effect of cysteamine and independence from repair functions of the host cell

Journal Article · · Radiobiology (USSR) (Engl. Transl.); (United States)
OSTI ID:5591168
; ; ;
A study was made of the lethal and mutagenic effects of /sup 60/Co ..gamma..-rays on lambda cI857 phage, when irradiated in nutrient broth in the absence and presence of 5.10/sup -2/ M cysteamine, using different repair-deficient E. coli mutants as hosts. It was found that cysteamine protection of extracellular phage from the lethal effect of ..gamma..-radiation is genetically determined, and that its effectiveness diminishes sharply with a change from a host of the wild type (DMF (dose modification factor) = 1.9) to repair-deficient rec A, lex A, pol A and uvr E mutants (DMF = 1.2 to 1.4). Induction of potentially postreplicative c mutations of phage is unrelated to repair mutations of the host cell, including the rec A and lex A mutations, which block cellular ..gamma..-mutagenesis. The hypothesis is expounded that induction of c mutations is related to ..gamma..-damaged DNA bases paired in error during vegetative replication of DNA. The presence of cysteamine does not affect the initial kinetics of ..gamma..-induced mutagenesis, but does increase the maximum incidence of c mutations.
Research Organization:
Inst. of Nuclear Physics, Leningrad, USSR
OSTI ID:
5591168
Journal Information:
Radiobiology (USSR) (Engl. Transl.); (United States), Journal Name: Radiobiology (USSR) (Engl. Transl.); (United States) Vol. 19:1; ISSN RADBA
Country of Publication:
United States
Language:
English

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Related Subjects

550700 -- Microbiology
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
BACTERIA
BACTERIOPHAGES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
COBALT 60
COBALT ISOTOPES
DNA
DNA REPLICATION
DRUGS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GAMMA RADIATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MEA
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
MUTAGENESIS
MUTANTS
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RECOVERY
REPAIR
RESPONSE MODIFYING FACTORS
THIOLS
VIRUSES
YEARS LIVING RADIOISOTOPES