Facilitation of allogeneic bone marrow engraftment in mice by total lymphoid irradiation combined with total-body irradiation

Journal Article · Fri Jul 01 04:00:00 EDT 1983 · Transplantation; (United States)

DOI:https://doi.org/10.1097/00007890-198307000-00003· OSTI ID:5585732

Ang, K K; Waer, M; van der Schueren, E; Vandeputte, M

Different groups of C57BL/Ka mice received daily fractions of 2 Gy total lymphoid irradiation (TLI) in a total dose of 34, 24, or 14 Gy. On the day after the last irradiation, 30 X 10(6) allogeneic (BALB/c) nucleated bone marrow cells were infused into the irradiated animals. When the last one or two fractions of the radiation schedule were given to the whole body (combined total lymphoid-total-body irradiation, TLBI): (1) stable bone marrow chimerism with a higher number of donor-type cells in the peripheral blood was induced in a higher percentage of mice that had received 34 Gy TLBI compared with mice that received 34 Gy TLI. (2) bone marrow chimerism could also be induced after 24 Gy or 14 Gy TLBI, whereas 24 and 14 Gy TLI alone were ineffective. The tolerance to the TLBI schedules was excellent and no clinical signs of graft-versus-host disease were noticed. It is concluded that the addition of TBI can facilitate bone marrow engraftment after TLI and drastically reduce the number of radiation fractions needed to obtain successful chimerism after allogeneic bone marrow transplantation in mice.

• Research Organization: Division of Experimental Radiotherapy, University Hospital St. Rafaeel, Leuven, Belgium
• OSTI ID: 5585732
• Journal Information: Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 36:1; ISSN TRPLA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CHIMERAS
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
EXTERNAL IRRADIATION
GRAFT-HOST REACTION
HEMATOPOIETIC SYSTEM
IMMUNOLOGY
IMMUNOSUPPRESSION
IRRADIATION
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MOSAICISM
ORGANS
RADIATION CHIMERAS
RADIOIMMUNOLOGY
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
WHOLE-BODY IRRADIATION