Allogeneic bone marrow transplantation in mice after total lymphoid irradiation: influence of breeding conditions and strain of recipient mice

Journal Article · Wed Feb 01 04:00:00 EST 1984 · J. Immunol.; (United States)

OSTI ID:5004001

Waer, M; Ang, K K; van der Schueren, E; Vandeputte, M

Different groups of C57BL/ka or BALB/c mice received a dose of 34 Gy or 42 Gy of fractionated total lymphoid irradiation (TLI) before bone marrow transplantation with 30 x 10/sup 6/ BALB/c or C57BL nucleated bone marrow cells, respectively. BALB/c mice that were not bred in specific pathogen-free conditions before TLI showed a high morbidity and mortality rate after 34 Gy of TLI and allogeneic bone marrow transplantation as compared with BALB/c or C57BL that were bred in pathogen-free conditions before irradiation. Many of the conventionally bred BALB/c mice had clinical and histologic signs of graft-vs-host disease after TLI and allogeneic bone marrow infusion. Although leucocytosis and lymphocytosis and the immunologic competence as measured with in vitro tests were equally depressed after 34 Gy TLI in BALB/c and C57BL mice, chimerism was nevertheless significantly easier to obtain in BALB/c mice. The incidence of chimerism after TLI could be enhanced in C57BL mice by increasing the total radiation dose from 34 to 42 Gy. This augmentation of chimerism was paralleled by the induction of more suppressor cells after 42 Gy of TLI in C57BL mice.

• Research Organization: Univ. of Leuven, Belgium
• OSTI ID: 5004001
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 132:2; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL BREEDING
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL VARIABILITY
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CHIMERAS
COBALT 60
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
EVALUATION
EXTERNAL IRRADIATION
FRACTIONATED IRRADIATION
GAMMA RADIATION
GENETIC VARIABILITY
HEMATOPOIETIC SYSTEM
IMMUNOSUPPRESSION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MINUTES LIVING RADIOISOTOPES
MOSAICISM
NUCLEI
ODD-ODD NUCLEI
ORGANS
PARTIAL BODY IRRADIATION
RADIATION CHIMERAS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RODENTS
SOMATIC CELLS
TISSUES
TRANSPLANTS
VERTEBRATES
YEARS LIVING RADIOISOTOPES