Transplantation of syngeneic transfected cells to probe the in vivo immune response to viral proteins

Journal Article · Tue Jan 01 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (USA)

OSTI ID:5584084

Nakayama, Hiroyuki; Shibata, Motohiro; Wohlenberg, C; Rooney, J F; Notkins, A L ^[1]

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1. National Institutes of Health, Bethesda, MD (USA)

BALB/3T3 cells were transfected with the glycoprotein D (gD) gene of herpes simplex virus (HSV) and a cell line expressing gD on the cell surface was isolated. In vitro, {sup 51}Cr release tests showed that the transfected cells were destroyed by anti-HSV antibody in the presence of complement. To investigate in vivo immune response, the gD-transfected cells were transplanted into the footpads of syngeneic HSV-immunized or unimmunized BALB/c mice. In unimmunized mice, transfected cells remained intact for 7 days or longer, and the site of injection showed only slight lymphocyte infiltration. In contrast, in immunized mice, transfected cells elicited massive lymphocyte infiltration and were mostly destroyed by day 4. Analysis of infiltrating cells revealed that they were mainly Thy1{sup +} and CD8{sup +} lymphocytes along with small numbers of CD5{sup +}, CD4{sup +}, and B lymphocytes. These studies show that transfected murine cells expressing gD can be used to study the in vivo immune response to single viral proteins and they argue that the immune response contributes to the pathogenesis of HSV infection.

• OSTI ID: 5584084
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (USA), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (USA) Vol. 5:1; ISSN FAJOE; ISSN 0892-6638
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GENES
GLYCOPROTEINS
HERPES SIMPLEX
IMMUNE REACTIONS
IMMUNOASSAY
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
NUCLEI
ORGANIC COMPOUNDS
PATHOGENESIS
PROTEINS
RADIOISOTOPES
RODENTS
SKIN DISEASES
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES
VIRAL DISEASES