Characterization of rapid recovery from. gamma. -ray damage in plateau-phase human diploid fibroblasts

Journal Article · Mon Feb 01 04:00:00 EST 1982 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575773· OSTI ID:5579503

Malcolm, A W; Tomkinson, K N; Little, J B

Rapid recovery was investigated in density-inhibited, stationary-phase human diploid cells subcultured to low density at various times after a single dose of radiation. The influence of total dose, postirradiation temperature, and cell-to-cell contact on recovery was examined. The cultures were exposed to /sup 60/Co ..gamma.. rays at a dose rate of 50 rad per second. When the irradiation and postirradiation temperatures were maintained at 25 or 37/sup 0/C, recovery, as manifested by an enhancement in survival, was similar during the first 30 min, whereas it was reduced at later times in the 25/sup 0/C compared with the 37/sup 0/C cultures. This result suggests the existence of different rapidly and slowly acting components to recovery. No recovery was noted at any time with incubation at 4/sup 0/C. The recovery observed in density-inhibited cultures was consistently greater than that in less dense cultures, suggesting an effect of cell-to-cell contact and cell cycle distribution. Following single doses of 200 to 900 rad, progressive enhancement in relative recovery occurred with increasing doses over a period of 2 to 90 min, illustrating the importance of the relationship of the dose to the rapid recovery process.

• Research Organization: Harvard Univ., Boston, MA
• OSTI ID: 5579503
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 89:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
COBALT 60
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
DOSE RATES
ELECTROMAGNETIC RADIATION
FIBROBLASTS
GAMMA RADIATION
INJURIES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
RADIATION EFFECTS
RADIATION INJURIES
RADIATIONS
RADIOISOTOPES
RECOVERY
SOMATIC CELLS
TEMPERATURE EFFECTS
YEARS LIVING RADIOISOTOPES