Rapid recovery in plateau-phase mammalian cells. [. gamma. radiation, fractionated irradiation]

Journal Article · Mon Oct 01 04:00:00 EDT 1979 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575114· OSTI ID:5639038

Malcolm, A W; Little, J B

Rapid split-dose and rapid single-dose recovery was examined in two rodent cell lines in culture: hamster lung fibroblasts (V-79) and contact-inhibited mouse embryo fibroblasts (10T1/2). Cell turnover in density-inhibited plateau-phase 10T1/2 cell cultures is very low as compared with V-79 cells. Plateau cultures were exposed to cobalt-60 ..gamma.. rays at 85 to 90 rad/sec; 0 to 90 min of incubation at 37/sup 0/C was allowed either between two exposures or following a single exposure before subculture at low density. A rapid phase of recovery occurred in plateau-phase 10T1/2 cell cultures occurring within 4 to 10 min after irradiation, whereas little recovery occurred during this time in the V-79 cell line. Rapid recovery was not observed in either cell line following irradiation during exponential growth. When plateau-phase 10T1/2 cells were irradiated with a total dose of 1100 rad given as two 550-rad doses with exposures separated by 10 min, survival was enhanced by a factor of 5 over that seen following a single 1100-rad dose. When 10 min was allowed to elapse after a single dose before subculture, a twofold enhancement in survival occurred. This rapid recovery appeared to result from an additive effect of split-dose and potentially lethal damage repair. When rapid split-dose recovery had occurred, however, little further enhancement in survival took place when cells were placed under conditions which favored recovery of additional potentially lethal damage.

• Research Organization: Harvard, Univ., Boston, MA
• OSTI ID: 5639038
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 80:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL GROWTH
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CELL KILLING
COBALT 60
COBALT ISOTOPES
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
CUMULATIVE RADIATION EFFECTS
DATA
DATA FORMS
DOSES
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FIBROBLASTS
FRACTIONATED IRRADIATION
GAMMA RADIATION
GROWTH
HAMSTERS
INFORMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MICE
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
SURVIVAL CURVES
VERTEBRATES
YEARS LIVING RADIOISOTOPES