Alterations in the metabolism of benzo(a)pyrene in syrian hamster embryo (SHE) cells pretreated with phenolic antioxidants
Inhibition of chemical- or raddiation-induced neoplasia has been observed in animals whose diets were supplemented with antioxidants commonly used as food additives. Inhibition of the carcinogenicity of benzo(a)pyrene (BaP) or of 7,12-dimenthylbenz(a)anthracene (DMBA) - in rats has been achieved by the addition of the phenolic antioxidants butylated hydroxyanisole (BHA) or butylated hydroxytoluene (BHT) to the diet. Our data suggest that in SHE cells antioxidants inhibit the overall metabolism of BaP to its various oxidized moieties including 7,8-diol- and 7,8,9,10-tetrol-BaP. A plausible explanation for our results with SHE cells is that the antioxidants interact directly with AHH, thus inhibiting AHH metabolic capacity. From analysis of nuclear material from SHE cells (+- antioxidants) incubated for 36 hours with BaP at 1 ..mu..g/ml, it is calculated that 4.6, 2.4 and 2.9 pmol BaP are bound to the DNA isolated from 10/sup 7/ nuclei of control, BHA-(20 ..mu..g/ml) and p-MP-(10 ..mu..g/ml) treated cultures, respectively.
- Research Organization:
- Los Alamos National Lab., NM (USA)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 5578308
- Report Number(s):
- LA-UR-83-3093; CONF-8310179-2; ON: DE84001723
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDITIVES
ADDUCTS
ANIMAL CELLS
ANIMALS
ANTIOXIDANTS
AROMATICS
BENZOPYRENE
CELL CULTURES
CENTRIFUGATION
CHO CELLS
CHROMATOGRAPHY
CONDENSED AROMATICS
DATA
DNA ADDUCTS
ELECTROMAGNETIC RADIATION
ENZYMES
EXPERIMENTAL DATA
FOOD
HAMSTERS
HYDROCARBONS
HYDROXY COMPOUNDS
INFORMATION
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METABOLISM
METABOLITES
MIXED-FUNCTION OXIDASES
NUMERICAL DATA
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PHENOLS
RADIATIONS
RODENTS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRACENTRIFUGATION
VERTEBRATES
X RADIATION