Mitomycin c-induced sister chromatid exchanges and classical chromosome lesions in cultured lymphocytes of saguinus oedipus oedipus and saguinus fuscicollis illigeri

Technical Report · Sat Dec 01 00:00:00 EST 1979

DOI:https://doi.org/10.2172/5578203· OSTI ID:5578203

Sayer, A. M.

Lymphocyte cultures from 10 tamarins of the cancer prone S. oedipus species and from 10 tamarins of the cancer resistant S. fuscicollis species were treated in vitro with the clastogen mitomycin C to determine whether there were differences in sensitivity to mutagen-induced cytogenetic lesions between the two species of tamarins or within S. oedipus animals. No differences in either spontaneous or mutagen-induced sister chromatid exchange were observed between S. oedipus and S. fuscicollis, but there was significant variability in induced sister chromatid exchange frequencies within S. oedipus. Further studies of animals related to the tamarin with the highest induced sister chromatid exchanges demonstrated that the same trait was present in lymphocytes of a sibling and the female parent. Induced classical chromosome lesions evaluated in cultures from these same animals showed the same increased response over other animals tested from either species. These data suggest that individual S. oedipus animals may have increased sensitivity to mutagen-induced cytogenetic lesions and that this sensitivity may be the expression of a genetic trait. Whether this sensitivity is related to the increased incidence of colon cancer in animals of the same species is not known, but chromosomal sensitivity has been demonstrated in cells from patientss with several genetic diseases, also characterized by an increased risk of cancer.

• Research Organization: Univ. of Tennessee, Knoxville, TN (United States)
• DOE Contract Number: EY-76-C-05-0033
• OSTI ID: 5578203
• Report Number(s): DOE/TIC-11099
• Resource Relation: Other Information: Thesis
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
CHROMOSOMAL ABERRATIONS
GENETIC VARIABILITY
LYMPHOCYTES
SISTER CHROMATID EXCHANGES
MITOMYCIN
BIOLOGICAL EFFECTS
CELL CULTURES
EXPERIMENTAL DATA
IN VITRO
ISOLATED VALUES
MONKEYS
SENSITIVITY
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
ANTIMITOTIC DRUGS
BIOLOGICAL MATERIALS
BIOLOGICAL VARIABILITY
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DATA
DATA FORMS
DRUGS
INFORMATION
LEUKOCYTES
MAMMALS
MATERIALS
MUTATIONS
NUMERICAL DATA
PRIMATES
SOMATIC CELLS
VERTEBRATES
550900* - Pathology
551000 - Physiological Systems
550400 - Genetics
550600 - Medicine