Interrenal histochemistry of acid-exposed brook trout, Salvelinus fontinalis (Mitchill). [Brook]

Journal Article · Sat Jan 01 04:00:00 EST 1977 · Gen. Comp. Endocrinol.; (United States)

DOI:https://doi.org/10.1016/0016-6480(77)90019-3· OSTI ID:5577432

Mudge, J E; Dively, J L; Neff, W H; Anthony, A

Acute exposure of brook trout to acid water (pH 4.0) caused a significant decrease in interrenal (adrenocortical cell) nuclear diameter, concomitant with an increase in the intensity of nuclear staining. Cytochemical analyses of Feulgen-DNA (F-DNA) of interrenal cell nuclei revealed these cells to be diploid and nonreplicating. There was no loss in DNA evidenced in interrenal nuclei of acid-stressed trout. In the same fish, there appeared to be a progressive decrease in Azure B-RNA levels measured cytopotometrically over a 3-to 24-hr-exposure interval. A marked but transient elevation in circulating cortisol levels occurred at 1-3 hr of exposure with a return to control levels after 24 hr of exposure. No visually discernible changes in Sudan Black B staining for lipids were evidenced after acute acid exposure. The findings support the conclusion that there is a marked inhibition of RNA synthesis and presumably of steroidogenesis in interrenal tissue following acute exposure of trout to low pH.

• OSTI ID: 5577432
• Journal Information: Gen. Comp. Endocrinol.; (United States), Journal Name: Gen. Comp. Endocrinol.; (United States) Vol. 31; ISSN GCENA
• Country of Publication: United States
• Language: English

Related Subjects

520200 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID RAIN
ACIDIFICATION
ACUTE EXPOSURE
ANIMALS
AQUATIC ORGANISMS
ATMOSPHERIC PRECIPITATIONS
CELL CONSTITUENTS
CELL NUCLEI
DNA
FISHES
HISTOLOGY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PH VALUE
POLLUTION
RAIN
RNA
TOXICITY
TROUT
VERTEBRATES
WATER POLLUTION