Histology and DNA cytophotometry of the pancreas of acid exposed brook trout (Salvelinus fontinalis)
The gross anatomy and histology of the pancreas were studied in untreated and acid exposed brook trout (Salvelinus fontinalis). Also, three fixatives (4% formalin, Zenker-formol, and Bouin's) and four staining procedures (hematoxylin and eosin, phloxine-methylene blue, Mallory-Heidenhain's azan, and chromium-hematoxylin-phloxine) were evaluated in terms of their utility for histological studies of the brook trout pancreas. Finally, the exocrine cells of control and acid exposed trout were examined cytophotometrically to determine feulgen DNA content. Briefly, the major findings were as follows: although pancreatic tissue in the brook trout is diffusely scattered among the pyloric caeca and mesenteries bordering hepatic, splenic and small intestinal areas, and bulk of the gland resides next to the pyloric stomach and its entrance into the small intestine; pancreatic islets are also most numerous and best developed in this region; endocrine islets are composed of two granular cell types: Basophilic beta cells which comprise more than 70% of the islet and alpha cells which are scattered singly throughout each islet; histochemical analysis of F-DNA content of exocrine pancreas under control and experimental conditions indicates that these cells were not influenced by acute acid exposure; no histopathological changes were found in either endocrine or exocrine cells which could be attributed to prior exposure of trout to acid pH; and Mallory-Heidenhain's azan staining of Zenker-formol fixed tissue specimens proved to be an excellent stain for histological studies of both endocrine and exocrine components of the trout pancreas.
- OSTI ID:
- 5602385
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACIDIFICATION
ANIMALS
AQUATIC ECOSYSTEMS
AQUATIC ORGANISMS
BIOLOGICAL STRESS
BODY
DIGESTIVE SYSTEM
DNA
ECOSYSTEMS
ENDOCRINE GLANDS
FISHES
GLANDS
HISTOLOGY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PATHOLOGICAL CHANGES
TROUT
VERTEBRATES