Analyses of pancreas development by generation of gfp transgenic zebrafish using an exocrine pancreas-specific elastaseA gene promoter

Journal Article · Mon May 15 04:00:00 EDT 2006 · Experimental Cell Research

DOI:https://doi.org/10.1016/j.yexcr.2006.01.016· OSTI ID:20775373

Haiyan, Wan ^[1]; Korzh, Svitlana ^[1]; Zhen, Li ^[1]; Mudumana, Sudha Puttur ^[1]; Korzh, Vladimir ^[1]; Yunjin, Jiang ^[2]; Shuo, Lin ^[3]; Zhiyuan, Gong ^[4]

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1. Department of Biological Sciences, National University of Singapore (Singapore)
2. Laboratory of Developmental Signaling and Patterning, Institute of Molecular and Cell Biology (Singapore)
3. Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, CA 90024 (United States)
4. Department of Biological Sciences, National University of Singapore (Singapore) and Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, CA 90024 (United States)

In contrast to what we know on development of endocrine pancreas, the formation of exocrine pancreas remains poorly understood. To create an animal model that allows observation of exocrine cell differentiation, proliferation, and morphogenesis in living animals, we used the zebrafish elastaseA (elaA) regulatory sequence to develop transgenic zebrafish that display highly specific exocrine pancreas expression of GFP in both larvae and adult. By following GFP expression, we found that the pancreas in early development was a relatively compact organ and later extended posterior along the intestine. By transferring the elaA:gfp transgene into slow muscle omitted mutant that is deficient in receiving Hedgehog signals, we further showed that Hedgehog signaling is required for exocrine morphogenesis but not for cell differentiation. We also applied the morpholino knockdown and toxin-mediated cell ablation approaches to this transgenic line. We showed that the development of exocrine pancreas is Islet-1 dependent. Injection of the diphtheria toxin A (DTA) construct under the elastaseA promoter resulted in selective ablation of exocrine cells while the endocrine cells and other endodermal derivatives (liver and intestine) were not affected. Thus, our works demonstrated the new transgenic line provided a useful experimental tool in analyzing exocrine pancreas development.

• OSTI ID: 20775373
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 9 Vol. 312; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ABLATION
CELL DIFFERENTIATION
DIPHTHERIA
GENES
GLUCAGON
INSULIN
INTESTINES
LARVAE
LIVER
MORPHOGENESIS
MUSCLES
MUTANTS
PANCREAS
PROLIFERATION
SOMATOSTATIN
TOXINS