Monitoring of environmental cancer initiators through hemoglobin adducts by a modified Edman degradation method
Journal Article
·
· Anal. Biochem.; (United States)
Tissue doses of cancer initiators/mutagens are suitably monitored through hemoglobin adducts formed in vivo, but the use of this method has been hampered by a lack of sufficiently simple and fast procedures. It was previously observed that when the N-terminal amino acid in hemoglobin, valine, is alkylated it is cleaved off by the Edman sequencing reagent, phenyl isothiocyanate, in the neutral-alkaline coupling medium, as opposed to the acidic medium required by normal amino acids. Based on this principle, conditions for a functioning procedure for gas chromatography/mass spectrometry (GC/MS) determination of N-terminal alkylvalines in hemoglobin were worked out. Derivatizing the protein in formamide solution with pentafluorophenyl isothiocyanate, using a /sup 2/H-alkylated protein as internal standard, and applying on-column injection during analysis, permit reproducible determination of hydroxyethylvaline and other adducts down into the dose range where cancer risks may be considered acceptably low.
- Research Organization:
- Univ. of Stockholm, Sweden
- OSTI ID:
- 5577005
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 1; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKYLATION
AMINO ACIDS
CARBOXYLIC ACIDS
CARCINOGENS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DEUTERIUM
DISEASES
GAS CHROMATOGRAPHY
GLOBIN
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MASS SPECTROSCOPY
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RISK ASSESSMENT
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
TRACER TECHNIQUES
VALINE
59 BASIC BIOLOGICAL SCIENCES
ALKYLATION
AMINO ACIDS
CARBOXYLIC ACIDS
CARCINOGENS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DEUTERIUM
DISEASES
GAS CHROMATOGRAPHY
GLOBIN
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MASS SPECTROSCOPY
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RISK ASSESSMENT
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
TRACER TECHNIQUES
VALINE