Comparison of ethylene oxide and 4-aminobiphenyl hemoglobin adducts in cigarette smokers

Conference · Thu Sep 01 04:00:00 EDT 1988 · Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA)

OSTI ID:7018281

Latriano, L; Perera, F; Brenner, D; Jeffrey, A M ^[1]

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1. Columbia Univ., New York, NY (USA)

The use of protein adducts as a dosimeter of exposure to chemical carcinogenesis was suggested by Ehrenberg et al who showed that the level of hemoglobin adducts was related to DNA adduct levels in target tissues. The original method for the detection of ethylene oxide hemoglobin adducts, which involved digesting the protein into its constituent amino acids, extensive chromatographic separation of the modified histidines from the vast excess of normal amino acids and finally gc/ms analysis of hydroxyethyl-histidine adducts, has been improved by the use of a modified Edman degradation reaction. This method selectively removes only alkylated N-terminal valine residues from hemoglobin. This approach has been successfully applied to the analysis of ethylene oxide adducts in the hemoglobin of cigarette smokers. Hemoglobin adducts of 4-aminobiphenyl (ABP) have also been used as a dosimeter for cigarette smoking. In this method, covalently bound ABP is hydrolyzed from the hemoglobin and the free ABP derivatized and analyzed by gc/ms. In the present study hemoglobin from smokers and non-smokers, which had previously been analyzed for 4-ABP by Tannenbaum et al, were analyzed for ethylene oxide adducts in order to determine whether the adduct levels of these two compounds could be correlated with cigarette consumption.

• OSTI ID: 7018281
• Report Number(s): CONF-8909236--
• Conference Information: Journal Name: Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA) Journal Volume: 28:2
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
AEROSOLS
ALKENES
AROMATICS
BIOSYNTHESIS
BIPHENYL
CARBOXYLIC ACIDS
CHROMATOGRAPHY
COLLOIDS
COMPARATIVE EVALUATIONS
DISPERSIONS
DNA ADDUCTS
ETHYLENE
GLOBINS
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RESIDUES
SEPARATION PROCESSES
SMOKES
SOLS
SYNTHESIS
TOBACCO SMOKES