Monitoring human exposure to ethylene oxide by the determination of hemoglobin adducts using gas chromatography-mass spectrometry

Journal Article · Tue Apr 01 04:00:00 EST 1986 · Carcinogenesis; (United States)

DOI:https://doi.org/10.1093/carcin/7.4.637· OSTI ID:5558260

Farmer, P B; Bailey, E; Gorf, S M; Toernqvist, M O; Osterman-Golkar, S; Kautiainen, A; Lewis-Enright, D P

Globin samples from ethylene oxide-exposed workers and non-exposed referrents were analyzed by two methods: (i) gas chromatography-mass spectrometry determination of Nt-(2-hydroxyethyl)histidine as its methyl ester heptafluorobutyryl derivative, after hydrolysis of the protein and isolation of the alkylated amino acid by ion exchange chromatography. The internal standard, Nt-(2-hydroxy-d4-ethyl)histidine, was added to the protein before hydrolysis. (ii) Determination of N-(2-hydroxyethyl)valine after derivatization of the protein by a modified Edman procedure, extraction and g.c.-m.s. determination of alkylated N-terminal valine in the form of its pentafluorophenylthiohydantoin derivative. The internal standard used was in this case a globin with a known content of hydroxy-d4-ethylated amino acids. The two methods gave consistent results, especially at high levels of alkylated products. The average content of hydroxyethylhistidine was 0.6 nmol/g higher than the content of hydroxyethylvaline. Higher levels of background alkylation (of unknown origin) were recorded with the histidine method as compared with the valine method, suggesting that the latter assay should show greater sensitivity for low level ethylene oxide exposure monitoring.

• Research Organization: MRC Toxicology Unit, Surrey, England
• OSTI ID: 5558260
• Journal Information: Carcinogenesis; (United States), Journal Name: Carcinogenesis; (United States) Vol. 4; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

560306* -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKENES
ALKYLATION
AMINO ACIDS
AZOLES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHROMATOGRAPHY
ETHYLENE
GAS CHROMATOGRAPHY
GLOBIN
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
HYDROCARBONS
IMIDAZOLES
ION EXCHANGE CHROMATOGRAPHY
MASS SPECTROSCOPY
OCCUPATIONAL EXPOSURE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PERSONNEL
PIGMENTS
PORPHYRINS
PROTEINS
SEPARATION PROCESSES
SPECTROSCOPY