Liver cells in culture: a model for investigating the regulation of retinol-binding protein metabolism

Journal Article · Tue Sep 30 04:00:00 EDT 1980 · Ann. N.Y. Acad. Sci.; (United States)

DOI:https://doi.org/10.1111/j.1749-6632.1980.tb29528.x· OSTI ID:5575420

Borek, C; Smith, J E; Goodman, D S

Vitamin A and its analogs influence a variety of parameters in cell growth and differentiation. The present discussion will be devoted to their action in vitro on differentiation and will concentrate on the regulation of the retinol binding protein by vitamin A. Retinol circulates in the plasma bound to retinol binding protein (RBP), which has been purified from a variety of species including humans and rats. RBP is a small protein with a molecular weight of about 20,000 daltons, which has a single binding site for one molecule of retinol. The secretion of RBP from the liver is regulated in a specific manner by the availability of retinol. Information on the secretion of RBP and its regulation has been obtained heretofore from in vivo studies. For this reason and for the purpose of investigating cellular and molecular mechanisms of RBP production and secretion, it seemed desirable to find an in vitro model of liver cells in culture that would produce RBP and be responsive to a variety of factors that regulate RBP in vivo.

• Research Organization: Columbia Univ., NY
• DOE Contract Number: EP-78-S-02-4733
• OSTI ID: 5575420
• Journal Information: Ann. N.Y. Acad. Sci.; (United States), Journal Name: Ann. N.Y. Acad. Sci.; (United States) Vol. 349; ISSN ANYAA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550500 -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMAL CELLS
BIOLOGICAL MODELS
BODY
CORTICOSTEROIDS
DEXAMETHASONE
DIGESTIVE SYSTEM
DISEASES
GLANDS
GLUCOCORTICOIDS
HEPATOMAS
HORMONES
HYDROXY COMPOUNDS
IN VITRO
KETONES
LIVER
METABOLISM
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PROTEINS
STEROID HORMONES
STEROIDS
TUMOR CELLS
VITAMIN A
VITAMINS