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Regulation of retinol-binding protein metabolism by glucocorticoid hormones in cultured H/sub 4/II EC/sub 3/ liver cells

Journal Article · · Endocrinology; (United States)
OSTI ID:6163537
; ; ;

Studies were conducted to explore the effects of glucocorticoid hormones on the regulation of the metabolism of retinol-binding protein (RBP) by H/sub 4/II EC/sub 4/ rat hepatoma cells in culture. Cortisol, corticosterone, and the synthetic glucocorticoid analog dexamethasone all induced a 2- to 3-fold increase in the accumulation of RBP. Half-maximal stimulation occurred at concentrations of dexamethasone in the range of 1 to 5 nM. Progesterone in the concentration range of 1 to 10 ..mu..M, inhibited the stimulatory effect of dexamethasone. Progesterone alone in this concentration range had no effect on RBP metabolism. The addition of retinol over a range from 3.5 nM to 3.5 ..mu..M stimulated a dose-dependent secretion of RBP from the cells into the medium. When studied over a wide range of concentrations, retinol and dexamethasone incubated together produced approximately additive increases in the accumulation of RBP. Dexamethasone, moreover, did not affect the retinol-induced secretion of RBP. Thus, retinol and dexamethasone appear to function via different and independent mechanisms to regulate the metabolism of RBP by the liver cell.

Research Organization:
Columbia Univ., New York, NY
DOE Contract Number:
EP-78-S-02-4733
OSTI ID:
6163537
Journal Information:
Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 109:2; ISSN ENDOA
Country of Publication:
United States
Language:
English

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Related Subjects

550200* -- Biochemistry
550500 -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
CHEMICAL ACTIVATION
CORTICOSTEROIDS
CORTICOSTERONE
DEXAMETHASONE
DISEASES
DOSE-RESPONSE RELATIONSHIPS
GLUCOCORTICOIDS
HEPATOMAS
HORMONES
HYDROXY COMPOUNDS
KETONES
MAMMALS
METABOLISM
NEOPLASMS
ORGANIC COMPOUNDS
PREGNANES
PROGESTERONE
PROTEINS
RATS
RODENTS
STEROID HORMONES
STEROIDS
VERTEBRATES
VITAMIN A
VITAMINS