Competitive enzyme immunoassay for human chorionic somatomammotropin using the avidin-biotin system
Journal Article
·
· Anal. Biochem.; (United States)
Human chorionic somatomammotropin (HCS) is determined by an enzyme immunoassay where HCS competes with biotin-labeled HCS for insolubilized anti-HCS antibodies. Enzyme-labeled avidin is then used to reveal the amount of bound HCS. The system proves to be sensitive (1 ng/ml of HCS can be detected) and results agree with radioimmunoassay determinations (correlation coefficient = 0.979). Kinetics of the avidin-biotin reaction and coating of polystyrene wells are also investigated.
- Research Organization:
- ISVT Sclava Research Center, Siena, Italy
- OSTI ID:
- 5574660
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 118:1; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200 -- Biochemistry
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
AZOLES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOTIN
CARBOXYLIC ACIDS
COMPARATIVE EVALUATIONS
ENZYME INHIBITORS
ENZYMES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
IMIDAZOLES
ISOTOPE APPLICATIONS
KINETICS
LIGANDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
REACTION KINETICS
STH
TRACER TECHNIQUES
VITAMIN B GROUP
VITAMINS
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
AZOLES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOTIN
CARBOXYLIC ACIDS
COMPARATIVE EVALUATIONS
ENZYME INHIBITORS
ENZYMES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
IMIDAZOLES
ISOTOPE APPLICATIONS
KINETICS
LIGANDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
REACTION KINETICS
STH
TRACER TECHNIQUES
VITAMIN B GROUP
VITAMINS