An enzyme immunoassay for plasma betamethasone

Journal Article · Sun Mar 01 04:00:00 EST 1981 · Steroids; (United States)

DOI:https://doi.org/10.1016/S0039-128X(81)90284-1· OSTI ID:5615222

Kominami, G; Yamauchi, A; Ishihara, S; Kono, M

A sensitive enzyme immunoassay for plasma betamethasone was developed using betamethasone-3-(O-carboxymethyl)oxime-beta-D-galactosidase conjugate as a labelled antigen and 4-methylumbelliferyl-beta-D-galactoside as a fluorescence substrate. The performances of the enzyme immunoassay were compared with that of a radioimmunoassay using /sup 3/H-betamethasone and the same antiserum. The minimal detectable level for the enzyme immunoassay was 0.15 pg/tube or 0.15 ng/ml of plasma, which was remarkably more sensitive than the radioimmunoassay level of 10 pg/tube or 2 ng/ml of plasma. The specificity was sufficient, in particular, the cross reactivity of cortisol as 0.008%. However, the precision of the enzyme immunoassay was inferior to that of the radioimmunoassay.

• OSTI ID: 5615222
• Journal Information: Steroids; (United States), Journal Name: Steroids; (United States) Vol. 31:3; ISSN STEDA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANTIGENS
BIOCHEMISTRY
CHEMISTRY
COMPARATIVE EVALUATIONS
CORTICOSTEROIDS
ENZYME ACTIVITY
FLUORESCENCE
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
LUMINESCENCE
ORGANIC COMPOUNDS
PREGNANES
RADIOASSAY
RADIOIMMUNOASSAY
SPECIFICITY
STEROID HORMONES
STEROIDS
SUBSTRATES
TRACER TECHNIQUES
TRITIUM COMPOUNDS