New radioimmunoassay technique using second antibody to solid phase applied to assays of human chorionic somatomammotropin

Journal Article · Mon Jan 01 04:00:00 EST 1973 · J. Nucl. Biol. Med., v. 17, no. 1, pp. 14-21

OSTI ID:4421861

Cocola, F; Genazzani, A R; Neri, P

A modification of the double antibody method for the radioimmunoassay of Human Chorionic-Somatomammotropln (HCS) is proposed. Rabbit antibodies raised against goat gamma globulin are coated on polystyrene tubes under suitable conditions, to separate the anti-HCS - HCS complex from the test mixture. Thus the specific antibody and the sample to be tested, or the standard hormone are placed successively in the coated tubes. After incubation at 37 deg C for four hours, the labeled hormone is added. This is followed by an addltional incubation at 37 deg C for fourteen hours. The precision and sensitivity of the method, and the good agreement with results obtained by the usual double antibody system, indicate that the Double Antibody-Solid Phase Radioimmunoassay (DASP-RIA) is a valuable improvement in the clinical assay of HCS, and suitable for wider application. (auth)

• Research Organization: Research Centre, Siena, Italy
• NSA Number: NSA-29-002982
• OSTI ID: 4421861
• Journal Information: J. Nucl. Biol. Med., v. 17, no. 1, pp. 14-21, Journal Name: J. Nucl. Biol. Med., v. 17, no. 1, pp. 14-21; ISSN JNBMA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*HORMONES-- RADIOIMMUNOASSAY
ANTIBODIES
IODINE 125
LABELLED COMPOUNDS
N48830* --Life Sciences--New Tracer Techniques--Other