Survival in soils of an herbicide-resistant Pseudomonas putida strain bearing a recombinant TOL plasmid

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Applied and Environmental Microbiology; (USA)

OSTI ID:5574467

Ramos, J L; Duque, E; Ramos-Gonzalez, M -I ^[1]

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1. Consejo Superior de Investigaciones Cientificas, Granada (Spain)

Pseudomonas putida EEZ15(pWW0-EB62) is a phosphinothricin (PPT)-resistant strain with a recombinant TOL plasmid which allows the strain to grown on p-ethylbenzoate. The survival of this strain in sterile agricultural soils depends on the physicochemical properties of the soil. The recombinant pWW0-EB62 plasmid and its catabolic functions were stable for periods of up to 1 month in bacteria introduced in unamended soils and only conferred selective advantage to the host bacteria without the plasmid or with the natural pWW0 plasmid when the soils were amended with low amounts of p-ethylbenzoate. The addition to soils of aromatics that are cometabolized by P. putida EEZ15(pWW0-EB62) had a detrimental effect on the survival of the bacteria, whereas low amounts of aromatics that are not metabolized by this bacterium had no effect on their survival. Survival of P. putida EEZ15(pWW0-EB62) was better at 4 and 25{degree}C than at 37{degree}C. The host bacterium carrying the recombinant pWW0-EB62 plasmid was established in unsterile soils.

• OSTI ID: 5574467
• Journal Information: Applied and Environmental Microbiology; (USA), Vol. 57:1; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
54 ENVIRONMENTAL SCIENCES
AROMATICS
TOXICITY
HERBICIDES
METABOLISM
PLASMIDS
PSEUDOMONAS
SOILS
TEMPERATURE EFFECTS
BACTERIA
CELL CONSTITUENTS
MICROORGANISMS
ORGANIC COMPOUNDS
PESTICIDES
553000* - Agriculture & Food Technology
560300 - Chemicals Metabolism & Toxicology
540210 - Environment
Terrestrial- Basic Studies- (1990-)