Genetic analysis of chromosomal operons involved in degradation of aromatic hydrocarbons in Pseudomonas putida TMB
- Universita degli Studi di Milano, Milan (Italy)
The catabolic pathway for the degradation of aromatic hydrocarbons encoded by Pseudomonas putida TMB differs from the TOL plasmid-encoded pathway as far as regulation of the upper pathway is concerned. We found, by analyzing Tn5-induced mutants and by Southern blot hybridization with appropriate probes derived from the TOL plasmid pWWO, that the catabolic genes of strain TMB were located on the bacterial chromosome and not on the 84-kb plasmid harbored by this strain. The catabolic genes of TMB and pWWO had sequence homology, as shown by Southern blot hybridization, but different significantly in their restriction patterns. The analysis of the mutants suggests that a regulatory mechanism similar to that present in pWWO coexists in TMB with a second mode of regulation which is epistatic on the former and that the chromosomal region carrying the catabolic genes is prone to rearrangements and deletions.
- OSTI ID:
- 5922052
- Journal Information:
- Journal of Bacteriology; (USA), Vol. 172:11; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PSEUDOMONAS
MOLECULAR BIOLOGY
TOLUENE
BIODEGRADATION
DNA HYBRIDIZATION
GENES
GENETICS
MUTANTS
PLASMIDS
ALKYLATED AROMATICS
AROMATICS
BACTERIA
BIOLOGY
CELL CONSTITUENTS
CHEMICAL REACTIONS
DECOMPOSITION
HYBRIDIZATION
HYDROCARBONS
MICROORGANISMS
ORGANIC COMPOUNDS
550400* - Genetics