Action of platelet-activating factor on type 1 diabetic human platelets

Journal Article · Mon Apr 01 04:00:00 EST 1985 · J. Lab. Clin. Med.; (United States)

OSTI ID:5565953

Greco, N J; Arnold, J H; O'Dorisio, T M; Cataland, S; Panganamala, R V

Platelets from patients with type 1 diabetes have exhibited more sensitivity to aggregation when compared with platelets from controls without diabetes after challenge with platelet-activating factor (PAF). The production of thromboxane B2 (TxB2) and 12-hydroxyeicosatetraenoic acid (12-HETE) and the release of 5-hydroxytryptamine (5HT) were increased when the platelets were challenged by PAF (5.0 x 10(-6) mol/L and 1.0 x 10(-6) mol/L). The production of TxB2 and 12-HETE and the release of 5HT were related to the irreversible biphasic aggregation profiles observed in the patients with diabetes. Inhibition of thromboxane A2 (TxA2) production by acetylsalicylic acid abolished the secondary wave of aggregation of platelets from patients with diabetes, changing an irreversible aggregation to a reversible one. Inhibition of both TxA2 and 12-HETE production by eicosatetraenoic acid did not contribute further to the inhibition caused by acetylsalicylic acid alone, indicating that 12-HETE was not involved in the secondary wave of aggregation. These data show that the increased aggregation observed in the platelets from the group with diabetes in response to PAF results in part from their higher production of TxA2 and release of 5HT.

• Research Organization: Ohio State Univ., Columbus
• OSTI ID: 5565953
• Journal Information: J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 105:4; ISSN JLCMA
• Country of Publication: United States
• Language: English

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550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLSALICYLIC ACID
AGGLOMERATION
ALDEHYDES
AMINES
ANALGESICS
ANTIPYRETICS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBOHYDRATES
CARBON ISOTOPES
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DIABETES MELLITUS
DISEASES
DRUGS
ENDOCRINE DISEASES
GLUCOSE
HETEROCYCLIC COMPOUNDS
HEXOSES
HYDROXY ACIDS
HYDROXY COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
METABOLIC DISEASES
MONOSACCHARIDES
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGY
PATIENTS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
SACCHARIDES
SEROTONIN
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRYPTAMINES