Platelet-activating factor increases platelet-dependent glycoconjugate secretion from tracheal submucosal gland

Journal Article · Fri Dec 01 04:00:00 EST 1989 · American Journal of Physiology; (USA)

OSTI ID:7168327

Sasaki, T; Shimura, S; Ikeda, K; Sasaki, H; Takishima, T ^[1]

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1. Tohoku Univ. School of Medicine, Sendai (Japan)

Using isolated glands from feline trachea, we examined the effect of platelet-activating factor (PAF) on radiolabeled glycoconjugate release and glandular contraction by measuring induced tension in the absence or presence of platelets. PAF alone did not produce any significant glandular contraction nor any significant change in glycoconjugate release from isolated glands. In the presence of purified platelets containing no plasma, PAF (10(-8) to 10(-5) M) produced significant glycoconjugate secretion in a dose-dependent fashion, but it produced no significant glandular contraction. PAF-evoked glycoconjugate secretion was time dependent, reaching a peak response of 277% of control 15-30 min after the exposure of isolated glands to 10(-5) M PAF in the presence of platelets and returning to 135% of controls at 2 h. Platelets alone did not produce any significant stimulation in glycoconjugate release. CV-3988, a known PAF antagonist, inhibited the secretory response to PAF. Methysergide, a known antagonist to receptors for 5-hydroxytryptamine, did not alter PAF-evoked glycoconjugate secretion. Both indomethacin and SQ 29,548, a thromboxane receptor antagonist, abolished the PAF-evoked glycoconjugate secretion from isolated submucosal glands. Epithiomethanothromboxane A2, a stable thromboxane A2 analogue, produced a significant increase in glycoconjugate secretion in a dose-dependent fashion. These findings indicate that PAF increases glycoconjugate release in the presence of platelets and that the increase is dependent on some aspect of platelet function, namely thromboxane generation.

• OSTI ID: 7168327
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 257; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BLOOD PLATELETS
BODY FLUIDS
CATS
COAGULANTS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
GLYCOPROTEINS
HEMATOLOGIC AGENTS
HYDRAZINE
HYDROGEN COMPOUNDS
IN VITRO
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
MUCOUS MEMBRANES
NITROGEN COMPOUNDS
ORGANIC COMPOUNDS
PROSTAGLANDINS
PROTEINS
REACTION KINETICS
RECEPTORS
RESPIRATORY SYSTEM
SECRETION
TIME DEPENDENCE
TRACER TECHNIQUES
TRACHEA
TRITIUM COMPOUNDS
VERTEBRATES