In vivo incorporation of (2-/sup 3/H)-myo-inositol into frog opsin
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
The in vivo incorporation of (2-/sup 3/H)-myo-inositol into frog retinal rod outer segment membranes was examined. About 25% of the recovered radioactivity was found to be protein-associated. Following acid hydrolysis of this material and extraction with hexane, all the radioactivity remained in the aqueous phase, indicating that the label was not in fatty acids. Following ion exchange column chromatography of the hydrolysate, the major radioactive compound comigrated on TLC with an internal standard of (U-/sup 14/C)-myo-inositol. SDS polyacrylamide gel electrophoresis of unextracted membranes indicated that the majority of the label was associated with opsin. These results indicate that (2-/sup 3/H)-myo-inositol was incorporated in vivo into opsin, presumably with retention of its chemical identity.
- Research Organization:
- Baylor College of Medicine, Houston, TX
- OSTI ID:
- 5562888
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 2; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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· Experimental Cell Research; (USA)
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OSTI ID:6250604
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BODY
BODY AREAS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHROMATOGRAPHY
DISTRIBUTION
ELECTROPHORESIS
ESTERS
EYES
FACE
FROGS
HEAD
IN VIVO
INOSITOL
INOSITOLS
ION EXCHANGE CHROMATOGRAPHY
LABELLED COMPOUNDS
LIPIDS
MEMBRANES
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
RETINA
SACCHARIDES
SENSE ORGANS
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
TISSUE DISTRIBUTION
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BODY
BODY AREAS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHROMATOGRAPHY
DISTRIBUTION
ELECTROPHORESIS
ESTERS
EYES
FACE
FROGS
HEAD
IN VIVO
INOSITOL
INOSITOLS
ION EXCHANGE CHROMATOGRAPHY
LABELLED COMPOUNDS
LIPIDS
MEMBRANES
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
RETINA
SACCHARIDES
SENSE ORGANS
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
TISSUE DISTRIBUTION
TRITIUM COMPOUNDS
VERTEBRATES