Transport and metabolism of indole-3-acetyl-myo-inositol-galactoside in seedlings of Zea mays

Journal Article · Mon Mar 31 23:00:00 EST 1986 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.80.4.961· OSTI ID:6991190

Komoszynski, M; Bandurski, R S

Indole-3-acetyl-myo-inositol galactoside labeled with /sup 3/H in the indole and /sup 14/C in the galactose moieties was applied to kernels of 5 day old germinating seedlings of Zea mays. Indole-3-acetyl-myo-inositol galactoside was not transported into either the shoot or root tissue as the intact molecule but was instead hydrolyzed to yield (/sup 3/H)indole-3-acetyl-myo-inositol and (/sup 3/H)indole-3-acetic acid which were then transported to the shoot with little radioactivity going to the root. With certain assumptions concerning the equilibration of applied (/sup 3/H)indole-3-acetyl-myo-inositol-(U-/sup 14/C)galactose with the endogenous pool, it may be concluded that indole-3-acetyl-myo-inositol galactoside in the endosperm supplies about 2 picomoles per plant per hour of indole-3-acetic acid to the shoot and thus is comparable to indole-3-acetyl-myo-inositol as a source of indoleacetic acid for the shoot. Quantitative estimates of the amount of galactose in the kernels suggest that (/sup 3/H)indole-3-acetyl-myo-inositol-(/sup 14/C) galactose is hydrolyzed after the compound leaves the endosperm but before it reaches the shoot. In addition, (/sup 3/H)indole-3-acetyl-myo-inositol-(/sup 14/C)galactose supplies appreciable amounts of /sup 14/C to the shoot and both /sup 14/C and /sup 3/H to an uncharacterized insoluble fraction of the endosperm.

• Research Organization: Michigan State Univ., East Lansing
• OSTI ID: 6991190
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 80:4; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
CEREALS
DISTRIBUTION
DOUBLE LABELLING
GRASS
GROWTH
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
MAIZE
METABOLISM
PLANT GROWTH
PLANT GROWTH REGULATORS
PLANTS
SEEDLINGS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS