Translocation of radiolabeled indole-3-acetic acid and indole-3-acetyl-myo-inositol from kernel to shoot of Zea mays L

Journal Article · Thu Dec 31 23:00:00 EST 1987 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.86.1.79· OSTI ID:6905650

Chisnell, J R; Bandurski, R S

Either 5-(/sup 3/H)indole-3-acetic acid (IAA) or 5-(/sup 3/H)indole-3-acetyl-myoinositol was applied to the endosperm of kernels of dark-grown Zea mays seedlings. The distribution of total radioactivity, radiolabeled indole-3-acetic acid, and radiolabeled ester conjugated indole-3-acetic acid, in the shoots was then determined. Differences were found in the distribution and chemical form of the radiolabeled indole-3-acetic acid in the shoot depending upon whether 5-(/sup 3/H)indole-3-acetic acid or 5-(/sup 3/H)indole-3-acetyl-myo-inositol was applied to the endosperm. We demonstrated that indole-3-acetyle-myo-inositol applied to the endosperm provides both free and ester conjugated indole-3-acetic acid to the mesocotyl and coleoptile. Free indole-3-acetic acid applied to the endosperm supplies some of the indole-3-acetic acid in the mesocotyl but essentially no indole-3-acetic acid to the coleoptile or primary leaves. It is concluded that free IAA from the endosperm is not a source of IAA for the coleoptile. Neither radioactive indole-3-acetyl-myo-inositol nor IAA accumulates in the tip of the coleoptile or the mesocotyl node and thus these studies do not explain how the coleoptile tip controls the amount of IAA in the shoot.

• Research Organization: Michigan State Univ., East Lansing (USA)
• OSTI ID: 6905650
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 86:1; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETIC ACID ESTERS
AROMATICS
AUXINS
AZAARENES
AZOLES
BODY
CARBOHYDRATES
CARBOXYLIC ACID ESTERS
CEREALS
ENDOSPERM
ESTERS
GRASS
GROWTH
HETEROCYCLIC COMPOUNDS
INDOLES
INOSITOLS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEAVES
MAIZE
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANT GROWTH
PLANT GROWTH REGULATORS
PLANT TISSUES
PLANTS
PRECURSOR
PYRROLES
SACCHARIDES
TISSUES
TRACER TECHNIQUES
TRANSLOCATION
TRITIUM COMPOUNDS