Morphological and biochemical studies of canine progressive rod-cone degeneration. /sup 3/H-fucose autoradiography

Journal Article · Thu May 01 04:00:00 EDT 1986 · Invest. Ophthalmol. Visual Sci.; (United States)

OSTI ID:5559904

Aguirre, G; O'Brien, P

Visual cell pathology and rod outer segment renewal were investigated in normal and PRCD-affected miniature poodles using /sup 3/H-fucose autoradiography. Twenty-four hours following the intravitreal injection of /sup 3/H-fucose, label accumulated diffusely over cone OS and in a banded pattern at the rod OS base. In normal rods, the band of /sup 3/H-label was displaced sclerad with time. PRCD-affected rods in the early stages of the disease (stages 0-1) also showed a similar /sup 3/H-label pattern but a significantly (P less than 0.001) reduced renewal rate (control = 2.35 +/- 0.43 mu/24 hr; affected = 0.99 +/- mu/24 hr). This abnormal renewal rate was present in central, equatorial, and peripheral visual cells and was not associated with the presence or density of pigment in the RPE cell layer. Biochemical studies indicated that the /sup 3/H-label was present as an integral membrane component in the rod OS and confirmed that canine rhodopsin is a fucosylated glycoprotein. The /sup 3/H-band in the rod OS layer disappeared in stage 2 of the disease; diffuse label now was present over rod OS that had decreased length and were reduced in number. At this stage of the disease, interphotoreceptor space was invaded by phagocytic cells, and photoreceptor nuclei were lost from the outer nuclear layer. These late degenerative changes were more extensive in the superior and inferior retinal meridians.

• Research Organization: Univ. of Pennsylvania, Philadelphia
• OSTI ID: 5559904
• Journal Information: Invest. Ophthalmol. Visual Sci.; (United States), Journal Name: Invest. Ophthalmol. Visual Sci.; (United States) Vol. 5; ISSN IOVSD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BODY
BODY AREAS
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
DISEASES
DOGS
EYES
FACE
HEAD
HEXOSES
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
RETINA
SACCHARIDES
SENSE ORGANS
SENSE ORGANS DISEASES
TRITIUM COMPOUNDS
VERTEBRATES