Cone outer segment shedding in the goldfish retina characterized with the /sup 3/H-fucose technique

Journal Article · Wed Sep 01 04:00:00 EDT 1982 · Invest. Ophthalmol. Visual Sci.; (United States)

OSTI ID:6580564

Balkema, Jr, G W; Bunt-Milam, A H

After an intravitreal injection of /sup 3/H-fucose, red- and blue-sensitive cone outer segments (OSs) in the goldfish retina became heavily labeled, green-sensitive cone OSs showed light labeling, and rod OSs showed virtually no labeling. Fish were maintained in white light (light/dark: 12 hr/12 hr; 6 to 10 weeks) and were injected with /sup 3/H-fucose 24 hr before sacrifice. After light onset, only phagosomes with no label were found in the retinal pigment epithelium (RPE); after light offset, phagosomes with heavy, light, or no label were found in the RPE. A broad peak of cone OS shedding derived from all cone types was found beginning 2 hr after light offset and returning to baseline levels after 12 hr, with a maximum at 4 to 6 hr. When the white light was replaced with red light during the final 24 hr (irradiance matched to the white light at 625 nm), the green cones showed a reduction in shedding by 62%, the rods showed a 48% reduction in shedding, and the number of heavily labeled phagosomes was reduced by 24% (a value that may reflect normal and red cone shedding and a reduction in blue cone shedding). The results suggest that chromatic stimulation during the light period may influence the shedding response of a given class of cone OS. Finally, the /sup 3/H-fucose technique is useful for determination of the photoreceptor OS from which a given phagosome in the RPE originates in this species.

• Research Organization: Department of Ophthalmology, University of Washington School of Medicine, Seattle
• OSTI ID: 6580564
• Journal Information: Invest. Ophthalmol. Visual Sci.; (United States), Journal Name: Invest. Ophthalmol. Visual Sci.; (United States) Vol. 23:3; ISSN IOVSD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AQUATIC ORGANISMS
BODY
BODY AREAS
CARBOHYDRATES
EYES
FACE
FISHES
GOLDFISH
HEAD
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MEDICINE
MONOSACCHARIDES
MORPHOLOGY
OPHTHALMOLOGY
ORGANIC COMPOUNDS
ORGANS
RETINA
SACCHARIDES
SENSE ORGANS
TRITIUM COMPOUNDS
VERTEBRATES