Proportion of antigenic variants induced by in vitro UV irradiation differs in clones derived from a single tumor

Journal Article · Fri Jan 15 04:00:00 EST 1988 · J. Immunol.; (United States)

OSTI ID:5556288

Hostetler, L W; Kripke, M L

The purpose of this study was to examine the capacity of different clones derived from the same tumor to generate highly antigenic cells after in vitro exposure to UV radiation. Cells from the metastatic murine melanoma K1735 and clones of K1735 differing in metastatic potential were exposed to UV radiation in vitro, cloned, and tested for antigenic properties in vivo. Approximately half of the clones isolated after UV irradiation of parental K1735 melanoma cells were highly antigenic (five of nine). Similar treatment of cells of a nonmetastatic clone of K1735 generated clones that were all antigenic (nine of nine). In contrast, only one of nine clones derived from UV-irradiated cells of a highly metastatic clone of K1735 were antigenic. Clones derived from unirradiated cultures were not antigenic variants. The increased antigenicity of cells derived from UV-irradiated cultures did not correlate with an increase in expression of cell surface class I major histocompatibility complex antigens. These results demonstrate that the frequency of antigenic variant production after UV irradiation is an intrinsic property of the particular cell line used, and that even cloned cell lines derived from a single tumor differ in their ability to generate antigenic variants after UV irradiation. In addition, they indicate that the increased antigenicity is not necessarily due to a UV-induced increase in expression of cell surface class I histocompatibility antigens.

• Research Organization: Univ. of Texas Cancer Center, Houston
• OSTI ID: 5556288
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 140:2; ISSN JOIMA
• Country of Publication: United States
• Language: English

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560120* -- Radiation Effects on Biochemicals
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