Histochemical applications of x-ray microanalysis: the simultaneous assessment of mitosis and cell death

Journal Article · Tue Jan 01 04:00:00 EST 1980 · Scanning Electron Microsc.; (United States)

OSTI ID:5550730

Bowen, I D; Lewis, G H

The principles of x-ray microanalytical histochemistry are reviewed. The use of labelling and precipitation techniques are examined, and particular attention is paid to the localization of enzymatic activity. A new method is described for the simultaneous assessment of mitosis as represented by the incorporation of (/sup 3/H) thymidine, and cell death as represented by the localization of free acid phosphatase, in the same tissue section. The thymidine incorporation is demonstrated by the appearance of topographically and microanalytically detectable silver grains in an overlying emulsion and the cell lysis associated acid phosphatase activity is detected optically and microanalytically by means of a bromine-rich azo dye deposited as a result of coupling naphthol AS BI, enzymatically released from naphthyl AS BI phosphoric acid, with diazotized 2,5-dibromoaniline.

• OSTI ID: 5550730
• Journal Information: Scanning Electron Microsc.; (United States), Journal Name: Scanning Electron Microsc.; (United States) Vol. 4; ISSN SEMYB
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AZINES
BIOLOGICAL LOCALIZATION
CELL DIVISION
CELL KILLING
CHEMICAL ANALYSIS
CHEMICAL COMPOSITION
CYTOLOGICAL TECHNIQUES
EFFICIENCY
ELECTROMAGNETIC RADIATION
ELECTRON MICROPROBE ANALYSIS
ENZYME ACTIVITY
HETEROCYCLIC COMPOUNDS
HISTOLOGY
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MEASURING METHODS
MICROANALYSIS
MITOSIS
NUCLEAR EMULSIONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
QUANTITATIVE CHEMICAL ANALYSIS
RADIATIONS
RIBOSIDES
SURVIVAL CURVES
THYMIDINE
TRACER TECHNIQUES
X RADIATION