Alkaline phosphatase in the intestine of the mouse during radioinduced atropy of the villi

Journal Article · Mon Jan 01 04:00:00 EST 1973 · Beitr. Pathol., v. 148, no. 2, pp. 180-198

OSTI ID:4404250

Luz, A; Goessner, W

The radiation-induced change of the functional epithelium the intestinal villi in the early postirradiation phase was studied the histochemical demonstration of an alkaline phosphatase as marker enzyme. Adult male mice received a single abdominal exposure to x irradiation of 1,200 rads. The animals were sacriiced between 1 and 10 days after irradiation. The histochemical demonstration of alkaline phosphatase with azo dye coupling techniques was applied to gross preparations of the formalin-calcium (5%C) fixed whole small intestine and to sections of the ileum. Alline phosphatase was simultaneously demonstrated with incorporated /sup 3/H-thymidine in autoradiograms according to EDER. Macroscopic and microscopic specimens show a complete loss of alkaline phosphatase activity in the villus epithellum of the ileum between 3 and 4 days after irradiation. The epithelial cells of the villi that are devoid of alkaline phosphatase also are not labelled with /sup 3/H-thymidine applied 1 hr before irradiation. The pattern of recurrence of enzyme active cells in the villi conforms to the pattern of cell regeneration observed with /sup 3/H-thymidine in the crypts at 4 to 5 days after irradiation. Seven days after irradiation the alkaline phosphatase reaction shows a normal picture in the ileum. (GE)

• Research Organization: Gesellschaft fuer Strahlen und Umweltforschung mbH, Nuremburg
• NSA Number: NSA-29-010451
• OSTI ID: 4404250
• Journal Information: Beitr. Pathol., v. 148, no. 2, pp. 180-198, Journal Name: Beitr. Pathol., v. 148, no. 2, pp. 180-198; ISSN BTPGA
• Country of Publication: Germany
• Language: German

Related Subjects

*ALKALINE PHOSPHATASE-- BIOCHEMISTRY
*SMALL INTESTINE-- BIOLOGICAL RADIATION EFFECTS
ABDOMEN
AUTORADIOGRAPHY
CRYPT CELLS
MICE
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates
PARTIAL BODY IRRADIATION
SOMATIC CELLS
THYMIDINE
TRITIUM COMPOUNDS
X RADIATION