sup 86 Rb(K) influx and ( sup 3 H)ouabain binding by human platelets: Evidence for beta-adrenergic stimulation of Na-K ATPase activity

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Metabolism, Clinical and Experimental; (USA)

DOI:https://doi.org/10.1016/0026-0495(89)90065-6· OSTI ID:5547276

Turaihi, K; Khokher, M A; Barradas, M A; Mikhailidis, D P; Dandona, P ^[1]

---

1. Royal Free Hospital and School of Medicine, London (England)

Although active transport of potassium into human platelets has been demonstrated previously, there is hitherto no evidence that human platelets have an ouabain-inhibitable Na-K ATPase in their membrane. The present study demonstrates active rubidium (used as an index of potassium influx), {sup 86}Rb(K), influx into platelets, inhibitable by ouabain, and also demonstrates the presence of specific ({sup 3}H)ouabain binding by the human platelet. This {sup 86}Rb(K) influx was stimulated by adrenaline, isoprenaline, and salbutamol, but noradrenaline caused a mild inhibition. Active {sup 86}Rb(K) influx by platelets was inhibited markedly by timolol, mildly by atenolol, but not by phentolamine. Therefore, active {sup 86}Rb(K) influx in human platelets is enhanced by stimulation of beta adrenoceptors of the beta 2 subtype. The platelet may therefore replace the leukocyte in future studies of Na-K ATPase activity. This would be a considerable advantage in view of the ease and rapidity of preparation of platelets.

• OSTI ID: 5547276
• Journal Information: Metabolism, Clinical and Experimental; (USA), Journal Name: Metabolism, Clinical and Experimental; (USA) Vol. 38:8; ISSN METAA; ISSN 0026-0495
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ADRENAL HORMONES
ADRENALINE
ALKALI METAL ISOTOPES
ALKALI METALS
ANIMALS
ATP-ASE
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
DRUGS
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GLYCOSIDES
HYDROGEN COMPOUNDS
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
MEMBRANES
METALS
MINUTES LIVING RADIOISOTOPES
NEUROREGULATORS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OUABAIN
PHOSPHOHYDROLASES
POTASSIUM
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RUBIDIUM 86
RUBIDIUM ISOTOPES
STROPHANTHINS
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES