Can maximum ouabain-sensitive /sup 86/Rb/sup +/ uptake be obtained by increasing Na/sup +/ influx
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6991719
Increasing stimulation frequency and monensin were utilized in attempts to enhance Na/sup +/ influx and thereby elicit the maximum rate of ouabain-sensitive /sup 86/Rb/sup +/ uptake in atrial muscle isolated from rat heart. Preparations were bathed in a K/sup +/-free, Rb/sup +/-containing Krebs-Henseleit buffer at 37/sup 0/C. Increasing stimulation frequency between 0 and 9 Hz enhanced the rate of ouabain-sensitive /sup 86/Rb/sup +/ uptake, whereas the uptake rate appeared to decrease at higher frequencies. Monensin (1.0 to 5.0 ..mu..M) increased the rate of uptake in atrial preparations stimulated at 6 Hz. A higher concentration of monensin produced a significant decline in uptake rate. The maximum rate of ouabain-sensitive /sup 86/Rb/sup +/ uptake produced by monensin in preparations stimulated at 6 Hz was not significantly different from the maximum elicited by stimulation frequency alone. The results suggest that either method of increasing Na/sup +/ influx may be used to maximize the rate of ouabain-sensitive /sup 86/Rb/sup +/ uptake and thereby estimate the Na/sup +/-pump capacity of rat atrial muscle.
- Research Organization:
- Univ. of Arkansas for Medical Sciences, Little Rock
- OSTI ID:
- 6991719
- Report Number(s):
- CONF-8604222-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
- Country of Publication:
- United States
- Language:
- English
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Journal Article
·
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·
OSTI ID:6207810
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DAYS LIVING RADIOISOTOPES
DRUGS
GLYCOSIDES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANE TRANSPORT
MINUTES LIVING RADIOISOTOPES
MUSCLES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OUABAIN
RADIOISOTOPES
RATS
RODENTS
RUBIDIUM 86
RUBIDIUM ISOTOPES
SODIUM COMPOUNDS
STROPHANTHINS
TRACER TECHNIQUES
UPTAKE
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DAYS LIVING RADIOISOTOPES
DRUGS
GLYCOSIDES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANE TRANSPORT
MINUTES LIVING RADIOISOTOPES
MUSCLES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OUABAIN
RADIOISOTOPES
RATS
RODENTS
RUBIDIUM 86
RUBIDIUM ISOTOPES
SODIUM COMPOUNDS
STROPHANTHINS
TRACER TECHNIQUES
UPTAKE
VERTEBRATES