Effect of reaerosolized fly ash from an atmospheric fluidized bed combustor on murine alveolar macrophages
Male B6CF/sub 1/ mice were exposed to reaerosolized fly ash. The fly ash was collected from the final filter of an atmospheric pressure fluidized-bed coal combustor and reaerosolized from a liquid nitrogen suspension. In four short experiments, (30-36 hrs; 6 hrs/day for 5-6 days) the fly ash was a composite of both start-up and steady-state material. In another experiment of longer duration (100 hrs; 6 hrs/day over a 4-week period), the fly ash used consisted of material from only steady-state operation of the combustor. Fly ash collected during steady-state operation is about 10 times less mutagenic in the Ames Salmonella test than is fly ash collected during start-up operation of the combustor. Mice exposed to fly ash in the short experiments showed a 2 to 4 fold increase in the number of alveolar macrophages 4 days after exposure and these macrophages were full of engulfed particles when examined by light microscopy. In in vitro tests 4-26 days after this exposure, macrophages from mice were unable to kill a challenge dose of Staphylococcus aureus as effectively as those from control mice. There was impairment in both phagocytic ability and in intracellular killing. Macrophages from mice exposed for 100 hrs to fly ash collected during steady-state operation were similarly full of engulfed particles when examined 4 days after exposure. However, the ability of these macrophages to kill S. aureus was unimpaired at this time and six months after exposure. These early observations suggest that the presence of mutagenic material on fly ash impairs the ability of macrophages to phagocytize and kill a challenge dose of S. aureus.
- Research Organization:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 5544151
- Report Number(s):
- CONF-791002-3
- Country of Publication:
- United States
- Language:
- English
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560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOL WASTES
AIR POLLUTION
ANIMAL CELLS
ANIMALS
BACTERIA
BIOLOGICAL EFFECTS
BODY
CHEMICAL REACTIONS
COMBUSTION
CONNECTIVE TISSUE CELLS
FLUIDIZED-BED COMBUSTION
FLY ASH
LUNGS
MACROPHAGES
MAMMALS
MICE
MICROORGANISMS
MUTAGENESIS
MUTAGENS
ORGANS
OXIDATION
PHAGOCYTES
PHAGOCYTOSIS
POLLUTION
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
STAPHYLOCOCCUS
START-UP
STEADY-STATE CONDITIONS
THERMOCHEMICAL PROCESSES
VERTEBRATES
WASTES