Turnover of cytokeratin polypeptides in mouse hepatocytes

Journal Article · Sun Nov 01 00:00:00 EST 1987 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(87)90339-9· OSTI ID:5534025

Denk, H; Lackinger, E; Zatloukal, K ^[1]; Franke, W W ^[2]

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1. Univ. of Graz (Austria)
2. German Cancer Research Center, Heidelberg (West Germany)

The turnover of cytokeratin polypeptides A (equivalent to No. 8 of the human cytokeratin catalog) and D (equivalent to human cytokeratin No. 18) of mouse hepatocytes was studied by pulse-labeling of mouse liver proteins after intraperitoneal injection of L-(guanido{sup 14}C)arginine and ({sup 14}C)sodium bicarbonate. With L-(guanido-{sup 14}C)arginine a rapid increase in the specific radioactivity of both cytokeratins was observed which reached a plateau between 12 and 24 h. With ({sup 14}C)sodium bicarbonate maximal specific radioactivity was obtained at 6 h followed by a rapid decrease to half maximum values within the subsequent 6 h and then a slower decrease. Half-lives were determined from the decrease of specific radioactivities after pulse-labeling by least-squares plots and found to be 84 h (for cytokeratin component A) and 104 h (component D) for arginine labeling . Values obtained after bicarbonate labeling were similar (95 h for A and 98 h for D). These results show that liver cytokeratins are relatively stable proteins and suggest that components A and D are synthesized and degraded at similar rates, probably in a coordinate way.

• OSTI ID: 5534025
• Journal Information: Experimental Cell Research; (United States), Vol. 173:1; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
KERATIN
METABOLISM
POLYPEPTIDES
BIOSYNTHESIS
ACID CARBONATES
ARGININE
CARBON 14 COMPOUNDS
LIVER CELLS
MICE
TRACER TECHNIQUES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
CARBON COMPOUNDS
CARBOXYLIC ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
PROTEINS
RODENTS
SCLEROPROTEINS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
550301* - Cytology- Tracer Techniques